Literature summary for 1.8.5.4 extracted from

Cherney, M.M.; Zhang, Y.; Solomonson, M.; Weiner, J.H.; James, M.N.
Crystal structure of sulfide:quinone oxidoreductase from Acidithiobacillus ferrooxidans: insights into sulfidotrophic respiration and detoxification (2010), J. Mol. Biol., 398, 292-305.

Search for more literature for 1.8.5.4

Cloned(Commentary)

Cloned (Comment)	Organism
-	Acidithiobacillus ferrooxidans

Crystallization (Commentary)

Crystallization (Comment)	Organism
native or in complex with decylubiquinone, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 600, 0.1 M 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol (pH 5.5 or pH 6.5), 0.1 M magnesium sulfate, and 0.05% (w/v) n-dodecyl beta-D-maltoside	Acidithiobacillus ferrooxidans

Protein Variants

Protein Variants	Comment	Organism
C128A	in the decylubiquinone assay, the mutant shows 30-35% activity compared to the wild type enzyme. However, in the FAD reduction assay, both the wild type and the Cys128Ala variant are fully active (100%)	Acidithiobacillus ferrooxidans
C160A	inactive	Acidithiobacillus ferrooxidans
C356A	inactive	Acidithiobacillus ferrooxidans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0028	-	Sulfide	in 50 mM 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol (pH 6.5), temperature not specified in the publication	Acidithiobacillus ferrooxidans
0.022	-	decylubiquinone	in 50 mM 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol (pH 6.5), temperature not specified in the publication	Acidithiobacillus ferrooxidans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Acidithiobacillus ferrooxidans	16020	-

Organism

Organism	UniProt	Comment	Textmining
Acidithiobacillus ferrooxidans	B7JBP8	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzymatic reaction catalyzed by sulfide:quinone oxidoreductase includes the oxidation of sulfide compounds H2S, HS-, and S2- to soluble polysulfide chains or to elemental sulfur in the form of octasulfur rings	Acidithiobacillus ferrooxidans	?	-	?
sulfide + decylubiquinone	-	Acidithiobacillus ferrooxidans	sulfur + decylubiquinol	-	?

Subunits

Subunits	Comment	Organism
dimer	x-ray crystallography	Acidithiobacillus ferrooxidans

Synonyms

Synonyms	Comment	Organism
sulfide:quinone oxidoreductase	-	Acidithiobacillus ferrooxidans

Cofactor

Cofactor	Comment	Organism	Structure
FAD	noncovalently bound	Acidithiobacillus ferrooxidans

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Release 2023.1 (January 2023)