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Literature summary for 1.8.5.4 extracted from

  • Marcia, M.; Langer, J.D.; Parcej, D.; Vogel, V.; Peng, G.; Michel, H.
    Characterizing a monotopic membrane enzyme. Biochemical, enzymatic and crystallization studies on Aquifex aeolicus sulfide:quinone oxidoreductase (2010), Biochim. Biophys. Acta, 1798, 2114-2123.
    View publication on PubMed
Search for more literature for 1.8.5.4

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Aquifex aeolicus
crystallization in two crystal forms of hexagonal, prism shape and thin, elongated shape Aquifex aeolicus

Inhibitors

Inhibitors Comment Organism Structure
Antimycin competitive Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 ubiquinone-4 pH 7.4, temperature not specified in the publication Aquifex aeolicus
0.0016
-
 ubiquinone-4 in 50 mM Tris-HCl, pH 7.4, 40°C Aquifex aeolicus
0.00216
-
 decylubiquinone pH 7.4, temperature not specified in the publication Aquifex aeolicus
0.00216
-
 decylubiquinone in 50 mM Tris-HCl, pH 7.4, 40°C Aquifex aeolicus
0.0054
-
 ubiquinone-1 pH 7.4, temperature not specified in the publication Aquifex aeolicus
0.0054
-
 ubiquinone-1 in 50 mM Tris-HCl, pH 7.4, 40°C Aquifex aeolicus
0.00594
-
 Sulfide pH 7.4, temperature not specified in the publication Aquifex aeolicus
0.00594
-
 Sulfide in 50 mM Tris-HCl, pH 7.4, 40°C Aquifex aeolicus
0.00643
-
 ubiquinone-9 pH 7.4, temperature not specified in the publication Aquifex aeolicus
0.00643
-
 ubiquinone-9 in 50 mM Tris-HCl, pH 7.4, 40°C Aquifex aeolicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane monotopic membrane enzyme Aquifex aeolicus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 sedimentation equilibrium centrifugation Aquifex aeolicus
47000
-
 x * 47000, SDS-PAGE Aquifex aeolicus
47000
-
 3 * 47000, SDS-PAGE Aquifex aeolicus
120000
-
 gel filtration Aquifex aeolicus
160000
-
 analytical ultracentrifugation Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-
Aquifex aeolicus O67931
-
-

Purification (Commentary)

Purification (Comment) Organism
MonoQ column chromatography, TSK-1 gel filtration, and TSK-2 gel filtration Aquifex aeolicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
71.4
-
 at 40°C, pH 7.4 Aquifex aeolicus

Storage Stability

Storage Stability Organism
4C, protein is stable and monodisperse in 50 mM dodecyl-D-maltoside for weeks, either in the absence of salt or in the presence of N 1 M NaCl Aquifex aeolicus
4°C, purified protein in 3% (w/v) dodecyl-beta-D-maltoside either in the absence of salt or in the presence of N 1 M NaCl, six weeks, no loss of activity Aquifex aeolicus
80°C, purified protein in 3% (w/v) dodecyl-beta-D-maltoside either in the absence of salt or in the presence of N 1 M NaCl, one day, 50% loss of activity Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfide + decylubiquinone
-
 Aquifex aeolicus sulfur + decylubiquinol
-
 ?
sulfide + ubiquinone-1
-
 Aquifex aeolicus sulfur + ubiquinol-1
-
 ?
sulfide + ubiquinone-4
-
 Aquifex aeolicus sulfur + ubiquinol-4
-
 ?
sulfide + ubiquinone-9
-
 Aquifex aeolicus sulfur + ubiquinol-9
-
 ?

Subunits

Subunits Comment Organism
homotrimer 3 * 47000, SDS-PAGE Aquifex aeolicus
multimer x * 47000, SDS-PAGE Aquifex aeolicus

Synonyms

Synonyms Comment Organism
sulfide:quinone oxidoreductase
-
 Aquifex aeolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Aquifex aeolicus
80
-
 the activity is highest at 80°C (the highest temperature tested) Aquifex aeolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 the half-life at 80°C is 32 h Aquifex aeolicus
80
-
 50% loss of activity after one day Aquifex aeolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Aquifex aeolicus

Cofactor

Cofactor Comment Organism Structure
FAD one FAD molecule per protein monomer Aquifex aeolicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.015
-
 in 50 mM Tris-HCl, pH 7.4, 40°C Aquifex aeolicus Antimycin