Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhodobacter capsulatus |
Protein Variants | Comment | Organism |
---|---|---|
C127S | 1.3% activity compared to the wild type enzyme | Rhodobacter capsulatus |
C159S | 0.5% activity compared to the wild type enzyme | Rhodobacter capsulatus |
C353S | 0.4% activity compared to the wild type enzyme | Rhodobacter capsulatus |
H131A | 20% activity at pH 6.5 and 27% activity at (optimum) pH 4.5 compared to the wild type enzyme | Rhodobacter capsulatus |
H196A | 38% activity at pH 6.5 and 40% activity at (optimum) pH 6.2 compared to the wild type enzyme | Rhodobacter capsulatus |
V300D | 11% activity compared to the wild type enzyme | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
decylubiquinone | mutant enzyme H196A, in 250 mM Tris-HCl (pH 8.0), temperature not specified in the publication | Rhodobacter capsulatus | |
0.003 | - |
decylubiquinone | wild type enzyme, in 250 mM Tris-HCl (pH 8.0), temperature not specified in the publication | Rhodobacter capsulatus | |
0.005 | - |
Sulfide | wild type enzyme, in 250 mM Tris-HCl (pH 8.0), temperature not specified in the publication | Rhodobacter capsulatus | |
0.011 | - |
Sulfide | mutant enzyme H131A, in 250 mM Tris-HCl (pH 8.0), temperature not specified in the publication | Rhodobacter capsulatus | |
0.015 | - |
Sulfide | mutant enzyme H196A, in 250 mM Tris-HCl (pH 8.0), temperature not specified in the publication | Rhodobacter capsulatus | |
0.027 | - |
decylubiquinone | mutant enzyme H131A, in 250 mM Tris-HCl (pH 8.0), temperature not specified in the publication | Rhodobacter capsulatus | |
0.028 | - |
decylubiquinone | Km above 0.028 mM, mutant enzyme V300D, in 250 mM Tris-HCl (pH 8.0), temperature not specified in the publication | Rhodobacter capsulatus | |
0.4 | - |
Sulfide | Km above 0.4 mM, mutant enzyme V300D, in 250 mM Tris-HCl (pH 8.0), temperature not specified in the publication | Rhodobacter capsulatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rhodobacter capsulatus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
- |
Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, gel filtration | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfide + decylubiquinone | - |
Rhodobacter capsulatus | sulfur + decylubiquinol | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Rhodobacter capsulatus |
Synonyms | Comment | Organism |
---|---|---|
SQR | - |
Rhodobacter capsulatus |
sulfide-quinone reductase | - |
Rhodobacter capsulatus |
sulfide:quinone oxidoreductase | - |
Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
- |
Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the only cofactor, noncovalently bound | Rhodobacter capsulatus |