Literature summary for 1.8.5.2 extracted from

Kikumoto, M.; Nogami, S.; Kanao, T.; Takada, J.; Kamimura, K.
Tetrathionate-forming thiosulfate dehydrogenase from the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans (2013), Appl. Environ. Microbiol., 79, 113-120.

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Activating Compound

Activating Compound	Comment	Organism	Structure
Sodium sulfate	the purified enzyme requires 150 mM sodium sulfate to generate maximum activity	Acidithiobacillus ferrooxidans
sulfite	the addition of sulfite (2 mM) results in the activation of the enzyme at a magnitude of 1.6fold	Acidithiobacillus ferrooxidans

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Acidithiobacillus ferrooxidans

Inhibitors

Inhibitors	Comment	Organism	Structure
Sodium sulfate	60% reduction in enzyme activity is observed in the reaction mixture containing 200 mM sodium sulfate	Acidithiobacillus ferrooxidans
sulfite	almost complete inhibition in the presence of 10 mM sulfite	Acidithiobacillus ferrooxidans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
8	-	thiosulfate	recombinant enzyme, at pH 2.5 and 50°C	Acidithiobacillus ferrooxidans
15	-	thiosulfate	native enzyme, at pH 2.5 and 70°C	Acidithiobacillus ferrooxidans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25000	-	x * 25000, SDS-PAGE	Acidithiobacillus ferrooxidans

Organism

Organism	UniProt	Comment	Textmining
Acidithiobacillus ferrooxidans	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, CM-650M column chromatography, butyl-650M column chromatography, and TSKgel G3000 gel filtration	Acidithiobacillus ferrooxidans

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture condition:sulfur-grown cell	-	Acidithiobacillus ferrooxidans	-
culture condition:tetrathionate-grown cell	-	Acidithiobacillus ferrooxidans	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.05	-	unpurified recombinant enzyme, at pH 2.5 and 40°C	Acidithiobacillus ferrooxidans
3.16	-	recombinant enzyme after 63.2fold purification, at pH 2.5 and 40°C	Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme reduces neither ubiquinone nor horse heart cytochrome c, which serves as an electron acceptor	Acidithiobacillus ferrooxidans	?	-	?
thiosulfate + ferricyanide + H+	when the concentration of thiosulfate is lower than 4 mM, no enzyme activity is detected	Acidithiobacillus ferrooxidans	tetrathionate + ferrocyanide	-	?

Subunits

Subunits	Comment	Organism
?	x * 25000, SDS-PAGE	Acidithiobacillus ferrooxidans

Synonyms

Synonyms	Comment	Organism
tetrathionate-forming thiosulfate dehydrogenase	-	Acidithiobacillus ferrooxidans
TSD	-	Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	recombinant enzyme	Acidithiobacillus ferrooxidans
70	-	native enzyme	Acidithiobacillus ferrooxidans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.5	-	native and recombinant enzyme	Acidithiobacillus ferrooxidans

pH Range

pH Minimum	pH Maximum	Comment	Organism
2	3	the native enzyme shows about 35% activity at pH 2.0 and 20% activity at pH 3.0, the recombinant enzyme shows about 80% activity at pH 2.0 and about 35% activity at pH 3.0	Acidithiobacillus ferrooxidans

Cofactor

Cofactor	Comment	Organism	Structure
additional information	does not contain heme	Acidithiobacillus ferrooxidans

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Release 2023.1 (January 2023)