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Literature summary for 1.8.5.2 extracted from
- Coupling of the pathway of sulphur oxidation to dioxygen reduction: characterization of a novel membrane-bound thiosulphate:quinone oxidoreductase (2004), Mol. Microbiol., 53, 1147-1160.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Dithionite | 1 mM, complete inhibition | Acidianus ambivalens |  |
| Metabisulfite | 1 mM, complete inhibition | Acidianus ambivalens | |
| additional information | no inhibition by sulfate or tetrathionate | Acidianus ambivalens | |
| N-ethylmaleimide | 1 mM, 54% inhibition | Acidianus ambivalens | |
| reduced titanium citrate | 1 mM, 65% inhibition | Acidianus ambivalens | |
| sulfite | 0.005 mM, 48% inhibition, 0.05 mM, complete inhibition | Acidianus ambivalens | |
| Triton X-100 | 1%, complete inhibition | Acidianus ambivalens | |
| Zn2+ | 1 mM, 37% inhibition, 5 mM, 82% inhibition | Acidianus ambivalens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00587 | - |
6-decylubiquinone | - |
Acidianus ambivalens | |
| 0.0059 | - |
6-decylubiquinone | 80°C, pH 6.0 | Acidianus ambivalens | |
| 2.6 | - |
thiosulfate | - |
Acidianus ambivalens | |
| 2.6 | - |
thiosulfate | 80°C, pH 6.0 | Acidianus ambivalens | |
| 3.4 | - |
ferricyanide | - |
Acidianus ambivalens | |
| 3.4 | - |
ferricyanide | 80°C, pH 6.0 | Acidianus ambivalens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Acidianus ambivalens | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | - |
alpha2,beta2, 2 * 16000 + 2 * 28000, SDS-PAGE | Acidianus ambivalens |
| 16000 | - |
alpha2beta2, the 28000 Da subunit and the 16000 Da subunit are identical to to DoxA and DoxD from Acidianus ambivalens quinol:oxygen oxidoreductase, 2 * 16000 + 2 * 28000, SDS-PAGE | Acidianus ambivalens |
| 18700 | - |
alpha2,beta2, 2 * 20400 + 2 * 18700, deduced from nucleotide sequence | Acidianus ambivalens |
| 20400 | - |
alpha2,beta2, 2 * 20400 + 2 * 18700, deduced from nucleotide sequence | Acidianus ambivalens |
| 28000 | - |
alpha2,beta2, 2 * 16000 + 2 * 28000, SDS-PAGE | Acidianus ambivalens |
| 28000 | - |
alpha2beta2, the 28000 Da subunit and the 16000 Da subunit are identical to to DoxA and DoxD from Acidianus ambivalens quinol:oxygen oxidoreductase, 2 * 16000 + 2 * 28000, SDS-PAGE | Acidianus ambivalens |
| 102000 | - |
gel filtration | Acidianus ambivalens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 thiosulfate + caldariellaquinone + 2 H+ | Acidianus ambivalens | the physiological electron acceptor is most probably a caldariella quinone type quinone | tetrathionate + caldariellaquinol | - |
r | |
| thiosulfate + 6-decylubiquinone | Acidianus ambivalens | the enzyme couples sulfur compound oxidation with quinone reduction | tetrathionate + 6-decylubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidianus ambivalens | P97224 AND P97207 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the larger subunit (28000 Da) appears to be glycosylated | Acidianus ambivalens |
| phosphoprotein | glycosylation of subunit DoxA, i.e. beta subunit of TQO | Acidianus ambivalens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acidianus ambivalens |
| solubilization, Q-Sepharose, hydroxylapatite, DEAE-Sepharose | Acidianus ambivalens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.397 | - |
activity with decyl ubiquinone at pH 6, 92°C | Acidianus ambivalens |
| 49.9 | - |
with ferricyanide as electron acceptor | Acidianus ambivalens |
| 73.4 | - |
activity with ferricyanide at pH 6, 92°C | Acidianus ambivalens |
| 397 | - |
with 6-decylubiquinone as electron acceptor | Acidianus ambivalens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 thiosulfate + caldariellaquinone + 2 H+ | the physiological electron acceptor is most probably a caldariella quinone type quinone | Acidianus ambivalens | tetrathionate + caldariellaquinol | - |
r | |
| additional information | horse heart cytochrome c is not reduced | Acidianus ambivalens | ? | - |
? | |
| tetrathionate + reduced methylene blue | - |
Acidianus ambivalens | thiosulfate + oxidized methylene blue | - |
r | |
| thiosulfate + 6-decylubiquinone | - |
Acidianus ambivalens | tetrathionate + 6-decylubiquinol | - |
? | |
| thiosulfate + 6-decylubiquinone | - |
Acidianus ambivalens | tetrathionate + 6-decylubiquinol | - |
r | |
| thiosulfate + 6-decylubiquinone | the enzyme couples sulfur compound oxidation with quinone reduction | Acidianus ambivalens | tetrathionate + 6-decylubiquinol | - |
? | |
| thiosulfate + ferricyanide | - |
Acidianus ambivalens | tetrathionate + ferrocyanide | - |
? | |
| thiosulfate + ferricyanide | - |
Acidianus ambivalens | tetrathionate + ferrocyanide | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha2,beta2, 2 * 16000 + 2 * 28000, SDS-PAGE | Acidianus ambivalens |
| tetramer | alpha2,beta2, 2 * 20400 + 2 * 18700, deduced from nucleotide sequence | Acidianus ambivalens |
| tetramer | alpha2beta2, the 28000 Da subunit and the 16000 Da subunit are identical to to DoxA and DoxD from Acidianus ambivalens quinol:oxygen oxidoreductase, 2 * 16000 + 2 * 28000, SDS-PAGE | Acidianus ambivalens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| thiosulfate oxidoreductase | - |
Acidianus ambivalens |
| thiosulfate oxidoreductase tetrathionate-forming | - |
Acidianus ambivalens |
| thiosulfate:quinone oxidoreductase | - |
Acidianus ambivalens |
| thiosulphate:quinone oxidoreductase | - |
Acidianus ambivalens |
| TQO | - |
Acidianus ambivalens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 92 | increasing activity is observed in the range between 20 and 92°C, a maximum is not observed because of technical reasons | Acidianus ambivalens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 92 | increasing activity is observed in the range between 20 and 92°C, a maximum is not observed because of technical reasons | Acidianus ambivalens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 167 | - |
thiosulfate | - |
Acidianus ambivalens | |
| 167 | - |
thiosulfate | 80°C, pH 6.0 | Acidianus ambivalens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | - |
Acidianus ambivalens |
| 5 | - |
- |
Acidianus ambivalens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 6.8 | approx. 55% of maximal activity at pH 3.5, approx. 22% of maximal activity at pH 6.5 | Acidianus ambivalens |
| 4.5 | 5.5 | pH 4.5: about 70% of maximal activity, pH 5.5: about 35% of maximal activity | Acidianus ambivalens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | a mixture of caldariella quinone, Sulfolobus quinone and menaquinone is non-covalently bound to the protein | Acidianus ambivalens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
sulfite | - |
Acidianus ambivalens | |
| 0.005 | - |
sulfite | pH 6.0, 80°C | Acidianus ambivalens | |
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