Literature summary for 1.8.5.1 extracted from

Do, H.; Kim, I.S.; Jeon, B.W.; Lee, C.W.; Park, A.K.; Wi, A.R.; Shin, S.C.; Park, H.; Kim, Y.S.; Yoon, H.S.; Kim, H.W.; Lee, J.H.
Structural understanding of the recycling of oxidized ascorbate by dehydroascorbate reductase (OsDHAR) from Oryza sativa L. japonica (2016), Sci. Rep., 6, 19498 .

Search for more literature for 1.8.5.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli NiCo21(DE3) cells	Oryza sativa Japonica Group

Crystallization (Commentary)

Crystallization (Comment)	Organism
native, ascorbate-bound, and glutathione-bound enzyme forms, hanging drop vapor diffusion method, using 0.15 M potassium bromide and 30% (w/v) PEG MME 2000	Oryza sativa Japonica Group

Protein Variants

Protein Variants	Comment	Organism
K20A	inactive	Oryza sativa Japonica Group
K47A	the mutant shows about wild type activity	Oryza sativa Japonica Group
K8A	the mutation significantly reduces the enzymatic activity	Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
glutathione + dehydroascorbate	Oryza sativa Japonica Group	-	glutathione disulfide + ascorbate	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa Japonica Group	Q65XA0	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 200 gel filtration	Oryza sativa Japonica Group

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathione + dehydroascorbate	-	Oryza sativa Japonica Group	glutathione disulfide + ascorbate	-	?

Subunits

Subunits	Comment	Organism
?	x * 23000, SDS-PAGE	Oryza sativa Japonica Group

Synonyms

Synonyms	Comment	Organism
dehydroascorbate reductase	-	Oryza sativa Japonica Group
DHAR	-	Oryza sativa Japonica Group

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	60	the enzyme retains more than 80% activity between 30 and 60°C for 20 min	Oryza sativa Japonica Group

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Release 2023.1 (January 2023)