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Literature summary for 1.8.5.1 extracted from

  • Zhang, Y.J.; Wang, W.; Yang, H.L.; Li, Y.; Kang, X.Y.; Wang, X.R.; Yang, Z.L.
    Molecular Properties and functional divergence of the dehydroascorbate reductase gene family in lower and higher plants (2015), PLoS ONE, 10, e0145038 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.5.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Zea mays
expressed in Escherichia coli BL21(DE3) cells Pinus taeda
expressed in Escherichia coli BL21(DE3) cells Picea abies
expressed in Escherichia coli BL21(DE3) cells Populus trichocarpa
expressed in Escherichia coli BL21(DE3) cells Selaginella moellendorffii
expressed in Escherichia coli BL21(DE3) cells Brachypodium distachyon
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
 dehydroascorbate isoform DHAR1, at pH 6.5 and 25°C Zea mays
0.068
-
 dehydroascorbate isoform DHAR2, at pH 6.5 and 25°C Zea mays
0.113
-
 dehydroascorbate isoform DHAR1, at pH 6.5 and 25°C Picea abies
0.115
-
 dehydroascorbate isoform DHAR1, at pH 6.5 and 25°C Selaginella moellendorffii
0.118
-
 dehydroascorbate isoform DHAR2, at pH 6.5 and 25°C Picea abies
0.119
-
 dehydroascorbate isoform DHAR4, at pH 6.5 and 25°C Zea mays
0.15
-
 dehydroascorbate isoform DHAR3, at pH 6.5 and 25°C Zea mays
0.248
-
 dehydroascorbate isoform DHAR2, at pH 6.5 and 25°C Selaginella moellendorffii
0.74
-
 glutathione isoform DHAR1, at pH 6.5 and 25°C Zea mays
1.079
-
 glutathione isoform DHAR3, at pH 6.5 and 25°C Zea mays
1.344
-
 glutathione isoform DHAR2, at pH 6.5 and 25°C Picea abies
1.466
-
 glutathione isoform DHAR1, at pH 6.5 and 25°C Selaginella moellendorffii
2.255
-
 glutathione isoform DHAR2, at pH 6.5 and 25°C Selaginella moellendorffii
2.507
-
 glutathione isoform DHAR2, at pH 6.5 and 25°C Zea mays
2.705
-
 glutathione isoform DHAR1, at pH 6.5 and 25°C Picea abies
2.883
-
 glutathione isoform DHAR4, at pH 6.5 and 25°C Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Zea mays 9507
-
chloroplast
-
 Pinus taeda 9507
-
chloroplast
-
 Picea abies 9507
-
chloroplast
-
 Populus trichocarpa 9507
-
chloroplast
-
 Selaginella moellendorffii 9507
-
chloroplast
-
 Brachypodium distachyon 9507
-
chloroplast
-
 Arabidopsis thaliana 9507
-
cytoplasm
-
 Zea mays 5737
-
cytoplasm
-
 Pinus taeda 5737
-
cytoplasm
-
 Picea abies 5737
-
cytoplasm
-
 Populus trichocarpa 5737
-
cytoplasm
-
 Selaginella moellendorffii 5737
-
cytoplasm
-
 Brachypodium distachyon 5737
-
cytoplasm
-
 Arabidopsis thaliana 5737
-
vacuole isoform DHAR3 Zea mays 5773
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione + dehydroascorbate Zea mays
-
 glutathione disulfide + ascorbate
-
 ?
glutathione + dehydroascorbate Pinus taeda
-
 glutathione disulfide + ascorbate
-
 ?
glutathione + dehydroascorbate Picea abies
-
 glutathione disulfide + ascorbate
-
 ?
glutathione + dehydroascorbate Populus trichocarpa
-
 glutathione disulfide + ascorbate
-
 ?
glutathione + dehydroascorbate Selaginella moellendorffii
-
 glutathione disulfide + ascorbate
-
 ?
glutathione + dehydroascorbate Brachypodium distachyon
-
 glutathione disulfide + ascorbate
-
 ?
glutathione + dehydroascorbate Arabidopsis thaliana
-
 glutathione disulfide + ascorbate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FWR4
-
-
Brachypodium distachyon
-
-
-
Picea abies
-
-
-
Pinus taeda
-
-
-
Populus trichocarpa
-
-
-
Selaginella moellendorffii
-
-
-
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose column chromatography Zea mays
Ni Sepharose column chromatography Pinus taeda
Ni Sepharose column chromatography Picea abies
Ni Sepharose column chromatography Populus trichocarpa
Ni Sepharose column chromatography Selaginella moellendorffii
Ni Sepharose column chromatography Brachypodium distachyon
Ni Sepharose column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Zea mays
-
leaf
-
 Pinus taeda
-
leaf
-
 Picea abies
-
leaf
-
 Populus trichocarpa
-
leaf
-
 Selaginella moellendorffii
-
leaf
-
 Brachypodium distachyon
-
leaf
-
 Arabidopsis thaliana
-
root
-
 Zea mays
-
root
-
 Pinus taeda
-
root
-
 Picea abies
-
root
-
 Populus trichocarpa
-
root
-
 Selaginella moellendorffii
-
root
-
 Brachypodium distachyon
-
root
-
 Arabidopsis thaliana
-
stem
-
 Zea mays
-
stem
-
 Pinus taeda
-
stem
-
 Picea abies
-
stem
-
 Populus trichocarpa
-
stem
-
 Selaginella moellendorffii
-
stem
-
 Brachypodium distachyon
-
stem
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.18
-
 isoform DHAR2, at pH 6.5 and 25°C Picea abies
0.22
-
 isoform DHAR2, at pH 6.5 and 25°C Selaginella moellendorffii
1.16
-
 isoform DHAR1, at pH 6.5 and 25°C Picea abies
3.22
-
 isoform DHAR4, at pH 6.5 and 25°C Zea mays
13.47
-
 isoform DHAR2, at pH 6.5 and 25°C Zea mays
18.45
-
 isoform DHAR1, at pH 6.5 and 25°C Zea mays
19.12
-
 isoform DHAR3, at pH 6.5 and 25°C Zea mays
26.15
-
 isoform DHAR1, at pH 6.5 and 25°C Selaginella moellendorffii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + dehydroascorbate
-
 Zea mays glutathione disulfide + ascorbate
-
 ?
glutathione + dehydroascorbate
-
 Pinus taeda glutathione disulfide + ascorbate
-
 ?
glutathione + dehydroascorbate
-
 Picea abies glutathione disulfide + ascorbate
-
 ?
glutathione + dehydroascorbate
-
 Populus trichocarpa glutathione disulfide + ascorbate
-
 ?
glutathione + dehydroascorbate
-
 Selaginella moellendorffii glutathione disulfide + ascorbate
-
 ?
glutathione + dehydroascorbate
-
 Brachypodium distachyon glutathione disulfide + ascorbate
-
 ?
glutathione + dehydroascorbate
-
 Arabidopsis thaliana glutathione disulfide + ascorbate
-
 ?

Synonyms

Synonyms Comment Organism
dehydroascorbate reductase
-
 Zea mays
dehydroascorbate reductase
-
 Pinus taeda
dehydroascorbate reductase
-
 Picea abies
dehydroascorbate reductase
-
 Populus trichocarpa
dehydroascorbate reductase
-
 Selaginella moellendorffii
dehydroascorbate reductase
-
 Brachypodium distachyon
dehydroascorbate reductase
-
 Arabidopsis thaliana
DHAR1 isoform Zea mays
DHAR1 isoform Pinus taeda
DHAR1 isoform Picea abies
DHAR1 isoform Populus trichocarpa
DHAR1 isoform Selaginella moellendorffii
DHAR1 isoform Brachypodium distachyon
DHAR1 isoform Arabidopsis thaliana
DHAR2 isoform Zea mays
DHAR2 isoform Pinus taeda
DHAR2 isoform Picea abies
DHAR2 isoform Populus trichocarpa
DHAR2 isoform Selaginella moellendorffii
DHAR2 isoform Brachypodium distachyon
DHAR2 isoform Arabidopsis thaliana
DHAR3 isoform Zea mays
DHAR4 isoform Zea mays

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 the enzyme retains more than 45% of its maximum enzymatic activity between 25 and 50°C Zea mays
25 50 the enzyme retains more than 45% of its maximum enzymatic activity between 25 and 50°C Pinus taeda
25 50 the enzyme retains more than 45% of its maximum enzymatic activity between 25 and 50°C Picea abies
25 50 the enzyme retains more than 45% of its maximum enzymatic activity between 25 and 50°C Populus trichocarpa
25 50 the enzyme retains more than 45% of its maximum enzymatic activity between 25 and 50°C Selaginella moellendorffii
25 50 the enzyme retains more than 45% of its maximum enzymatic activity between 25 and 50°C Brachypodium distachyon
25 50 the enzyme retains more than 45% of its maximum enzymatic activity between 25 and 50°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Zea mays
8
-
-
 Pinus taeda
8
-
-
 Picea abies
8
-
-
 Populus trichocarpa
8
-
-
 Selaginella moellendorffii
8
-
-
 Brachypodium distachyon
8
-
-
 Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10 the enzyme almost completely loses its activity below pH 5.5 or above pH 10.0 Zea mays
5.5 10 the enzyme almost completely loses its activity below pH 5.5 or above pH 10.0 Pinus taeda
5.5 10 the enzyme almost completely loses its activity below pH 5.5 or above pH 10.0 Picea abies
5.5 10 the enzyme almost completely loses its activity below pH 5.5 or above pH 10.0 Populus trichocarpa
5.5 10 the enzyme almost completely loses its activity below pH 5.5 or above pH 10.0 Selaginella moellendorffii
5.5 10 the enzyme almost completely loses its activity below pH 5.5 or above pH 10.0 Brachypodium distachyon
5.5 10 the enzyme almost completely loses its activity below pH 5.5 or above pH 10.0 Arabidopsis thaliana