Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells, Arabidopsis thaliana, and Nicotiana benthamiana | Actinidia chinensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
dehydroascorbate | isoform DHAR2, at pH 7.8 and 25°C | Actinidia chinensis | |
0.11 | - |
dehydroascorbate | isoform DHAR1, at pH 7.8 and 25°C | Actinidia chinensis | |
1.27 | - |
glutathione | isoform DHAR1, at pH 7.8 and 25°C | Actinidia chinensis | |
2.22 | - |
glutathione | isoform DHAR2, at pH 7.8 and 25°C | Actinidia chinensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isoform DHAR1 | Actinidia chinensis | 9507 | - |
cytosol | isoform DHAR1 | Actinidia chinensis | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + dehydroascorbate | Actinidia chinensis | - |
glutathione disulfide + ascorbate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinidia chinensis | - |
cultivar Hongyang | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Actinidia chinensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Actinidia chinensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + dehydroascorbate | - |
Actinidia chinensis | glutathione disulfide + ascorbate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, isoform DHAR1, SDS-PAGE | Actinidia chinensis |
? | x * 32000, isoform DHAR2, SDS-PAGE | Actinidia chinensis |
Synonyms | Comment | Organism |
---|---|---|
dehydroascorbate reductase | - |
Actinidia chinensis |
DHAR1 | isoform | Actinidia chinensis |
DHAR2 | isoform | Actinidia chinensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 55 | when preincubated at 35°C for 15 min, the enzyme activity is almost unaffected but begins to decrease when the temperature sets above 40°C. Isoform DHAR1 and DHAR2 still retain 61 and 73% of their initial activity when pre-incubated at 55°C for 15 min | Actinidia chinensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Actinidia chinensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | isoforms DHAR1 and DHAR2 retain more than 80% of their maximum enzymatic activities at pH values between 6.0 and 10.0, respectively | Actinidia chinensis |