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Literature summary for 1.8.4.9 extracted from

  • Phartiyal, P.; Kim, W.S.; Cahoon, R.E.; Jez, J.M.; Krishnan, H.B.
    The role of 5-adenylylsulfate reductase in the sulfur assimilation pathway of soybean: molecular cloning, kinetic characterization, and gene expression (2008), Phytochemistry, 69, 356-364.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Glycine max

Inhibitors

Inhibitors Comment Organism Structure
AMP competitive inhibitor with respect to 5'-adenylyl sulfate Glycine max
additional information sulfite (up to 100 mM) has no inhibitory effect Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0112
-
5'-adenylyl sulfate 30┬░C, pH 8.5, cosubstrate: dithiothreitol Glycine max
0.0174
-
5'-adenylyl sulfate 30┬░C, pH 8.5, cosubstrate: glutathione Glycine max
0.495
-
glutathione 30┬░C, pH 8.5 Glycine max
0.595
-
dithiothreitol 30┬░C, pH 8.5 Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
2 * 46000, SDS-PAGE Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
L. Merr., cv Williams 82
-

Purification (Commentary)

Purification (Comment) Organism
-
Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
root cold-treatment increases expression and the total activity of APS reductase in root tissues Glycine max
-
seed sulfur and phosphorus deprivation increases expression level, while nitrogen starvation represses APS reductase mRNA transcript and protein levels Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.207
-
-
Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-adenylyl sulfate + glutathione
-
Glycine max AMP + sulfite + glutathione disulfide
-
?
5'-adenylylsulfate + dithiothreitol
-
Glycine max adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol
-
?

Subunits

Subunits Comment Organism
dimer 2 * 46000, SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
5'-adenylylsulfate reductase
-
Glycine max
APS reductase
-
Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.4
-
glutathione 30┬░C, pH 8.5 Glycine max
8.4
-
5'-adenylyl sulfate 30┬░C, pH 8.5, cosubstrate: dithiothreitol Glycine max
10.1
-
dithiothreitol 30┬░C, pH 8.5 Glycine max
11.3
-
5'-adenylyl sulfate 30┬░C, pH 8.5, cosubstrate: glutathione Glycine max

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1
-
AMP
-
Glycine max