Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | competitive inhibitor with respect to 5'-adenylyl sulfate | Glycine max | |
additional information | sulfite (up to 100 mM) has no inhibitory effect | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0112 | - |
5'-adenylyl sulfate | 30°C, pH 8.5, cosubstrate: dithiothreitol | Glycine max | |
0.0174 | - |
5'-adenylyl sulfate | 30°C, pH 8.5, cosubstrate: glutathione | Glycine max | |
0.495 | - |
glutathione | 30°C, pH 8.5 | Glycine max | |
0.595 | - |
dithiothreitol | 30°C, pH 8.5 | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
2 * 46000, SDS-PAGE | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
L. Merr., cv Williams 82 | - |
Purification (Comment) | Organism |
---|---|
- |
Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | cold-treatment increases expression and the total activity of APS reductase in root tissues | Glycine max | - |
seed | sulfur and phosphorus deprivation increases expression level, while nitrogen starvation represses APS reductase mRNA transcript and protein levels | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.207 | - |
- |
Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-adenylyl sulfate + glutathione | - |
Glycine max | AMP + sulfite + glutathione disulfide | - |
? | |
5'-adenylylsulfate + dithiothreitol | - |
Glycine max | adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 46000, SDS-PAGE | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
5'-adenylylsulfate reductase | - |
Glycine max |
APS reductase | - |
Glycine max |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.4 | - |
glutathione | 30°C, pH 8.5 | Glycine max | |
8.4 | - |
5'-adenylyl sulfate | 30°C, pH 8.5, cosubstrate: dithiothreitol | Glycine max | |
10.1 | - |
dithiothreitol | 30°C, pH 8.5 | Glycine max | |
11.3 | - |
5'-adenylyl sulfate | 30°C, pH 8.5, cosubstrate: glutathione | Glycine max |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
AMP | - |
Glycine max |