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Literature summary for 1.8.4.9 extracted from

  • Suter, M.; von Ballmoos, P.; Kopriva, S.; Op den Camp, R.; Schaller, J.; Kuhlemeier, C.; Schurmann, P.; Brunold, C.
    Adenosine 5'-phosphosulfate sulfotransferase and adenosine 5'-phosphosulfate reductase are identical enzymes (2000), J. Biol. Chem., 275, 930-936.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Lemna minor

General Stability

General Stability Organism
enzyme unstable during purification and storage, addition of 5 mM 5'-adenosine monophosphate stabilizes activity Lemna minor

Inhibitors

Inhibitors Comment Organism Structure
5'-adenylylsulfate at 0.12 mM Lemna minor
adenosine-5'-phosphate competitive inhibition, Ki of 0.34 mM with 10 mM glutathione Lemna minor
glutathione at 60 mM Lemna minor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0065
-
5'-adenylylsulfate with 10 mM glutathione as reductant Lemna minor
0.0122
-
5'-adenylylsulfate with 40 mM glutathione as reductant Lemna minor
16.8
-
glutathione with 0.04 mM adenylylsulfate Lemna minor

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast sequence contains chloroplast transit peptide Lemna minor 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
SDS-PAGE Lemna minor
43640
-
calculated from cDNA sequence Lemna minor
91300
-
estimation by gel filtration with Superose 12 Lemna minor

Organism

Organism UniProt Comment Textmining
Lemna minor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification to homogeneity Lemna minor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.02 0.025 crude extract, plants treated with 10 mM NH4Cl Lemna minor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenosine-5'-phosphosulfate + glutathione no activity with 3'-phosphoadenosine-5'-phosphosulfate Lemna minor adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
?
5'-adenylylsulfate + dithioerythritol
-
Lemna minor adenosine 5'-monophosphate + sulfite + oxidized dithioerythritol
-
?
5'-adenylylsulfate + glutathione
-
Lemna minor adenosine monophosphate + sulfite + oxidized glutathione
-
?

Subunits

Subunits Comment Organism
dimer alpha2, 2 * 43000, comparison of native mass after gel filtration with denatured after SDS-PAGE Lemna minor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
56
-
5'-adenylylsulfate
-
Lemna minor