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Literature summary for extracted from

  • Suter, M.; von Ballmoos, P.; Kopriva, S.; Op den Camp, R.; Schaller, J.; Kuhlemeier, C.; Schurmann, P.; Brunold, C.
    Adenosine 5'-phosphosulfate sulfotransferase and adenosine 5'-phosphosulfate reductase are identical enzymes (2000), J. Biol. Chem., 275, 930-936.
    View publication on PubMed


Cloned (Comment) Organism
Lemna minor

General Stability

General Stability Organism
enzyme unstable during purification and storage, addition of 5 mM 5'-adenosine monophosphate stabilizes activity Lemna minor


Inhibitors Comment Organism Structure
5'-adenylylsulfate at 0.12 mM Lemna minor
adenosine-5'-phosphate competitive inhibition, Ki of 0.34 mM with 10 mM glutathione Lemna minor
glutathione at 60 mM Lemna minor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5'-adenylylsulfate with 10 mM glutathione as reductant Lemna minor
5'-adenylylsulfate with 40 mM glutathione as reductant Lemna minor
glutathione with 0.04 mM adenylylsulfate Lemna minor


Localization Comment Organism GeneOntology No. Textmining
chloroplast sequence contains chloroplast transit peptide Lemna minor 9507

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
SDS-PAGE Lemna minor
calculated from cDNA sequence Lemna minor
estimation by gel filtration with Superose 12 Lemna minor


Organism UniProt Comment Textmining
Lemna minor

Purification (Commentary)

Purification (Comment) Organism
purification to homogeneity Lemna minor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.02 0.025 crude extract, plants treated with 10 mM NH4Cl Lemna minor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenosine-5'-phosphosulfate + glutathione no activity with 3'-phosphoadenosine-5'-phosphosulfate Lemna minor adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
5'-adenylylsulfate + dithioerythritol
Lemna minor adenosine 5'-monophosphate + sulfite + oxidized dithioerythritol
5'-adenylylsulfate + glutathione
Lemna minor adenosine monophosphate + sulfite + oxidized glutathione


Subunits Comment Organism
dimer alpha2, 2 * 43000, comparison of native mass after gel filtration with denatured after SDS-PAGE Lemna minor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
Lemna minor