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Literature summary for 1.8.4.9 extracted from

  • Setya, A.; Murillo, M.; Leustek, T.
    Sulfate reduction in higher plants: Molecular evidence for a novel 5'-adenylylsulfate reductase (1996), Proc. Natl. Acad. Sci. USA, 93, 13383-13388.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
(NH4)2SO4 less effective than Na2SO4 Arabidopsis thaliana
DTT DTT can replace glutathione as reductant, 5 mM DTT increases activity 40fold Arabidopsis thaliana
Na2SO4
-
Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
-
Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
adenosine-5'-phosphate
-
Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast sequence contains chloroplast transit peptide Arabidopsis thaliana 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45600
-
clone APR2, calculated from cDNA sequence Arabidopsis thaliana
47300
-
clone APR3, calculated from cDNA sequence Arabidopsis thaliana
50800
-
clone APR1, calculated from cDNA sequence Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
0.000007
-
with 3'-phosphoadenosine 5'-phosphosulfate as substrate and 5 mM DTT, recombinant protein expressed in Escherichia coli Arabidopsis thaliana
0.00022
-
with 5'-adenylylsulfate as substrate and 5 mM DTT, recombinant protein expressed in Escherichia coli Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenosine-5'-phosphosulfate + glutathione 50fold lower activity than with 5'-adenylylsulfate Arabidopsis thaliana adenosine 3',5'-diphosphate + sulfite + oxidized glutathione
-
?
5'-adenylylsulfate + dithiothreitol
-
Arabidopsis thaliana adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol
-
?
5'-adenylylsulfate + glutathione
-
Arabidopsis thaliana adenosine monophosphate + sulfite + oxidized glutathione
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
recombinant protein, expressed in Escherichia coli Arabidopsis thaliana