BRENDA - Enzyme Database
show all sequences of 1.8.4.8

Characterization and reconstitution of a 4Fe-4S adenylyl sulfate/phosphoadenylyl sulfate reductase from Bacillus subtilis

Berndt, C.; Lillig, C.H.; Wollenberg, M.; Bill, E.; Mansilla, M.C.; de Mendoza, D.; Seidler, A.; Schwenn, J.D.; J. Biol. Chem. 279, 7850-7855 (2004)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene cysH, expression in Escherichia coli strain BL21(DE3)
Bacillus subtilis
General Stability
General Stability
Organism
activity is lost upon exposure to air or upon dilution
Bacillus subtilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0064
-
3'-phosphoadenylyl-sulfate
recombinant enzyme, pH 8.0, with thioredoxin 1
Bacillus subtilis
0.0107
-
3'-phosphoadenylyl-sulfate
recombinant enzyme, pH 8.0, with glutaredoxin 1
Bacillus subtilis
0.0225
-
glutaredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate
Bacillus subtilis
0.0287
-
3'-phosphoadenylyl sulfate
recombinant enzyme, pH 8.0, with thioredoxin 1
Bacillus subtilis
0.0543
-
thioredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate
Bacillus subtilis
0.0606
-
glutaredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate
Bacillus subtilis
0.0675
-
thioredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate
Bacillus subtilis
0.1054
-
3'-phosphoadenylyl sulfate
recombinant enzyme, pH 8.0, with glutaredoxin 1
Bacillus subtilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
the enzyme contains a [4Fe-4S] cluster per subunit, absolutely required for activity, constitution is altered in presence of oxygen, activity is restored after decomposition and reassembly
Bacillus subtilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
2 * 27000, SDS-PAGE
Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3'-adenylyl-sulfate + thioredoxin
Bacillus subtilis
-
adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
-
?
3'-phosphoadenylyl-sulfate + glutaredoxin
Bacillus subtilis
-
adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite
-
-
?
3'-phosphoadenylyl-sulfate + thioredoxin
Bacillus subtilis
-
adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Bacillus subtilis
-
gene cysH, bispecific enzyme
-
Oxidation Stability
Oxidation Stability
Organism
activity is lost upon exposure to air
Bacillus subtilis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3'-adenylyl-sulfate + thioredoxin
-
659441
Bacillus subtilis
adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
-
-
?
3'-phosphoadenylyl-sulfate + glutaredoxin
-
659441
Bacillus subtilis
adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite
-
-
-
?
3'-phosphoadenylyl-sulfate + thioredoxin
-
659441
Bacillus subtilis
adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 27000, SDS-PAGE
Bacillus subtilis
Synonyms
Synonyms
Commentary
Organism
4Fe-4S adenylyl sulfate/phosphoadenylyl sulfate reductase
-
Bacillus subtilis
CysH1
-
Bacillus subtilis
SNR
-
Bacillus subtilis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.146
-
glutaredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate
Bacillus subtilis
0.149
-
3'-phosphoadenylyl sulfate
recombinant enzyme, pH 8.0, with thioredoxin 1
Bacillus subtilis
0.172
-
3'-phosphoadenylyl-sulfate
recombinant enzyme, pH 8.0, with thioredoxin 1
Bacillus subtilis
0.174
-
thioredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate
Bacillus subtilis
0.219
-
thioredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate
Bacillus subtilis
0.335
-
3'-phosphoadenylyl sulfate
recombinant enzyme, pH 8.0, with glutaredoxin 1
Bacillus subtilis
0.382
-
3'-phosphoadenylyl-sulfate
recombinant enzyme, pH 8.0, with glutaredoxin 1
Bacillus subtilis
0.421
-
glutaredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate
Bacillus subtilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus subtilis
Cofactor
Cofactor
Commentary
Organism
Structure
glutaredoxin
glutaredoxin 1 variant
Bacillus subtilis
thioredoxin
thioredoxin 1 variant
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene cysH, expression in Escherichia coli strain BL21(DE3)
Bacillus subtilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
glutaredoxin
glutaredoxin 1 variant
Bacillus subtilis
thioredoxin
thioredoxin 1 variant
Bacillus subtilis
General Stability (protein specific)
General Stability
Organism
activity is lost upon exposure to air or upon dilution
Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0064
-
3'-phosphoadenylyl-sulfate
recombinant enzyme, pH 8.0, with thioredoxin 1
Bacillus subtilis
0.0107
-
3'-phosphoadenylyl-sulfate
recombinant enzyme, pH 8.0, with glutaredoxin 1
Bacillus subtilis
0.0225
-
glutaredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate
Bacillus subtilis
0.0287
-
3'-phosphoadenylyl sulfate
recombinant enzyme, pH 8.0, with thioredoxin 1
Bacillus subtilis
0.0543
-
thioredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate
Bacillus subtilis
0.0606
-
glutaredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate
Bacillus subtilis
0.0675
-
thioredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate
Bacillus subtilis
0.1054
-
3'-phosphoadenylyl sulfate
recombinant enzyme, pH 8.0, with glutaredoxin 1
Bacillus subtilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
the enzyme contains a [4Fe-4S] cluster per subunit, absolutely required for activity, constitution is altered in presence of oxygen, activity is restored after decomposition and reassembly
Bacillus subtilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
2 * 27000, SDS-PAGE
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3'-adenylyl-sulfate + thioredoxin
Bacillus subtilis
-
adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
-
?
3'-phosphoadenylyl-sulfate + glutaredoxin
Bacillus subtilis
-
adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite
-
-
?
3'-phosphoadenylyl-sulfate + thioredoxin
Bacillus subtilis
-
adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
-
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
activity is lost upon exposure to air
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3'-adenylyl-sulfate + thioredoxin
-
659441
Bacillus subtilis
adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
-
-
?
3'-phosphoadenylyl-sulfate + glutaredoxin
-
659441
Bacillus subtilis
adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite
-
-
-
?
3'-phosphoadenylyl-sulfate + thioredoxin
-
659441
Bacillus subtilis
adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 27000, SDS-PAGE
Bacillus subtilis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.146
-
glutaredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate
Bacillus subtilis
0.149
-
3'-phosphoadenylyl sulfate
recombinant enzyme, pH 8.0, with thioredoxin 1
Bacillus subtilis
0.172
-
3'-phosphoadenylyl-sulfate
recombinant enzyme, pH 8.0, with thioredoxin 1
Bacillus subtilis
0.174
-
thioredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate
Bacillus subtilis
0.219
-
thioredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate
Bacillus subtilis
0.335
-
3'-phosphoadenylyl sulfate
recombinant enzyme, pH 8.0, with glutaredoxin 1
Bacillus subtilis
0.382
-
3'-phosphoadenylyl-sulfate
recombinant enzyme, pH 8.0, with glutaredoxin 1
Bacillus subtilis
0.421
-
glutaredoxin 1
recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate
Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bacillus subtilis
Other publictions for EC 1.8.4.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742273
Berndt
-
The specificity of thioredoxi ...
Escherichia coli
Chem. Sci.
6
7049-7058
2015
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9
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1
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1
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10
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712502
Bhave
Spectroscopic studies on the [ ...
Mycobacterium tuberculosis
J. Biol. Chem.
286
1216-1226
2011
-
1
1
-
1
-
-
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1
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3
-
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1
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1
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3
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1
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2
2
698950
Chung
Interaction domain on thioredo ...
Pseudomonas aeruginosa
J. Biol. Chem.
284
31181-31189
2009
-
-
1
-
-
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1
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4
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1
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2
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685145
Chartron
3-Phosphoadenosine-5-phosphosu ...
Escherichia coli
Biochemistry
46
3942-3951
2007
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
1
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-
-
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1
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-
-
-
-
670348
Mansouri-Bauly
Sulfur uptake in the ectomycor ...
Laccaria bicolor, Laccaria bicolor S238N
Mycorrhiza
16
421-427
2006
1
-
-
-
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-
1
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9
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659441
Berndt
Characterization and reconstit ...
Bacillus subtilis
J. Biol. Chem.
279
7850-7855
2004
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1
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1
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8
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1
1
3
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2
1
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1
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3
1
3
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8
1
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8
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3
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8
1
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659214
Lillig
Redox regulation of 3'-phospho ...
Escherichia coli
J. Biol. Chem.
278
22325-22330
2003
1
-
1
-
1
-
1
-
-
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3
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2
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1
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1
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1
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3
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1
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1
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393953
Kopriva
The presence of an iron-sulfur ...
Escherichia coli
J. Biol. Chem.
277
21786-21791
2002
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1
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1
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2
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12
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1
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3
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3
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659140
Lillig
New thioredoxins and glutaredo ...
Escherichia coli
J. Biol. Chem.
274
7695-7698
1999
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-
1
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6
-
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2
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4
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1
1
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1
2
1
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1
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1
2
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6
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2
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1
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1
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5
1
1
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1
1
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133777
Montoya
Crystallization and preliminar ...
Escherichia coli
Acta Crystallogr. Sect. D
54
281-283
1998
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1
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133776
Savage
Crystal structure of phosphoad ...
Escherichia coli
Structure
5
895-906
1997
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1
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133778
Berendt
Reaction mechanism of thioredo ...
Escherichia coli
Eur. J. Biochem.
233
347-356
1995
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3
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4
2
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2
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1
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1
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133779
Niehaus
Primary structure of Synechoco ...
Synechococcus sp., Synechococcus sp. PCC 7942
Plant Mol. Biol.
20
1179-1183
1992
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1
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25
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1
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1
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133780
Krone
Characterisation of the gene c ...
Escherichia coli
Mol. Gen. Genet.
225
314-319
1991
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133775
Schwenn
Yeast PAPS reductase: properti ...
Saccharomyces cerevisiae
Arch. Microbiol.
150
313-319
1988
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