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Literature summary for 1.8.4.7 extracted from
- Purification and properties of a new glutathione-dependent thiol:disulphide oxidoreductase from rat liver (1982), Biochem. J., 207, 133-138.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | insensitive against 1 mM CuSO4 and 1 mM N-ethylmaleimide at 37°C for 30 min | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
1 * 40000, SDS-PAGE | Rattus norvegicus |
| 43000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
newborn and adults | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion-exchange, gel filtration | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| [xanthine dehydrogenase] + glutathione disulfide = [xanthine oxidase] + 2 glutathione | thioltransferase, GSH:insulin transhydrogenase and ricin-disulphide-bond reductase activity | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | highest amount of activity | Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| skeletal muscle | - |
Rattus norvegicus | - |
| skin | - |
Rattus norvegicus | - |
| spleen | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
372 U/mg, other definition of units | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | other disulfide compounds are either inactive or far less active than oxidized glutathione, reduction of disulfide bonds in ricin, thioltransferase activity, GSH:insulin transhydrogenase activity | Rattus norvegicus | ? | - |
? |  |
| [xanthine dehydrogenase] + oxidized glutathione | conversion into oxidase by treatment with proteolytic enzymes, storage at -20°C/-25°C or preincubation with either thiol reagents or whole liver homogenate. Can act in the opposite direction in the reduction of the disulfide bond of insulin and ricin | Rattus norvegicus | [xanthine oxidase] + glutathione | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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