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Literature summary for 1.8.4.16 extracted from
- Identification and charactcerization of a new disulfide isomerase-like protein (DsbD) in Escherichia coli (1995), EMBO J., 14, 3415-3424 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Escherichia coli | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a [protein] with reduced L-cysteine residues + thioredoxin disulfide | Escherichia coli | overall reaction | a [protein] carrying a disulfide bond + thioredoxin | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a [protein] with reduced L-cysteine residues + thioredoxin disulfide | overall reaction | Escherichia coli | a [protein] carrying a disulfide bond + thioredoxin | - |
? | |
| insulin + dithiothreitol | - |
Escherichia coli | reduced insulin + oxidized dithiothreitol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, calculated from amino acid sequence | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| disulfide isomerase-like protein | - |
Escherichia coli |
| DsbD | - |
Escherichia coli |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Escherichia coli | transcription of the enzyme gene is decreased at 50°C, but not completely turned off | down |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | enzyme loss leads to hypersensitivity to dithiothreitol and benzylpenicillin | Escherichia coli |
| metabolism | the enzyme generates a reducing source in the periplasm, which is required for maintaining proper redox conditions | Escherichia coli |
| physiological function | the enzyme is essential for bacterial growth at temperatures above 42°C | Escherichia coli |
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