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Literature summary for 1.8.4.14 extracted from

  • Gruez, A.; Libiad, M.; Boschi-Muller, S.; Branlant, G.
    Structural and biochemical characterization of free methionine-R-sulfoxide reductase from Neisseria meningitidis (2010), J. Biol. Chem., 285, 25033-25043.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.4.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Neisseria meningitidis

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with methionine sulfoxide, to 1.25 A resolution. Residue C118 is the catalytic Cys on which a sulfenic acid is formed Neisseria meningitidis

Protein Variants

Protein Variants Comment Organism
C118A inactive Neisseria meningitidis
C136A about 60% of the catalytic efficiency of wild-type Neisseria meningitidis
C84A about 120% of the catalytic efficiency of wild-type Neisseria meningitidis
C84A/C136A catalytic efficiency similar to wild-type Neisseria meningitidis
C84A/C94A/C136A about 35% of the catalytic efficiency of wild-type Neisseria meningitidis
C94A about 40% of the catalytic efficiency of wild-type Neisseria meningitidis
C94A/C136A about 35% of the catalytic efficiency of wild-type Neisseria meningitidis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 thioredoxin mutant C94A/C136A, 25°C, pH 8.0 Neisseria meningitidis
0.016
-
 thioredoxin mutant C84A/C94A/C136A, 25°C, pH 8.0 Neisseria meningitidis
0.024
-
 thioredoxin mutant C94A , 25°C, pH 8.0 Neisseria meningitidis
0.027
-
 thioredoxin mutant C136A, 25°C, pH 8.0 Neisseria meningitidis
0.04
-
 thioredoxin mutant C84A/C136A, 25°C, pH 8.0 Neisseria meningitidis
0.04
-
 thioredoxin wild-type, 25°C, pH 8.0 Neisseria meningitidis
0.05
-
 thioredoxin mutant C84A, 25°C, pH 8.0 Neisseria meningitidis
2
-
 L-methionine (R)-S-oxide wild-type, 25°C, pH 8.0 Neisseria meningitidis
2.2
-
 L-methionine (R)-S-oxide mutant C136A, 25°C, pH 8.0 Neisseria meningitidis
12
-
 L-methionine (R)-S-oxide mutant C84A/C136A, 25°C, pH 8.0 Neisseria meningitidis
18
-
 L-methionine (R)-S-oxide mutant C94A/C136A, 25°C, pH 8.0 Neisseria meningitidis
30
-
 L-methionine (R)-S-oxide mutant C84A, 25°C, pH 8.0 Neisseria meningitidis
30
-
 L-methionine (R)-S-oxide mutant C84A/C94A/C136A, 25°C, pH 8.0 Neisseria meningitidis
32
-
 L-methionine (R)-S-oxide mutant C94A , 25°C, pH 8.0 Neisseria meningitidis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18166
-
 2 * 18166, calculated Neisseria meningitidis
36330
-
 mass spectrometry under non-denaturing conditions Neisseria meningitidis

Organism

Organism UniProt Comment Textmining
Neisseria meningitidis A0A0Y6TLH6 serogroup C
-
Neisseria meningitidis 8013 A0A0Y6TLH6 serogroup C
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine (R)-S-oxide + thioredoxin
-
 Neisseria meningitidis L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine (R)-S-oxide + thioredoxin
-
 Neisseria meningitidis 8013 L-methionine + thioredoxin disulfide + H2O
-
 ?
additional information in the absence of thioredoxin, 1 mol of Met/subunit is formed Neisseria meningitidis ?
-
 ?
additional information in the absence of thioredoxin, 1 mol of Met/subunit is formed Neisseria meningitidis 8013 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 18166, calculated Neisseria meningitidis

Synonyms

Synonyms Comment Organism
NMV_2074 locus name Neisseria meningitidis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 L-methionine (R)-S-oxide mutant C84A/C94A/C136A, 25°C, pH 8.0 Neisseria meningitidis
1
-
 L-methionine (R)-S-oxide mutant C94A , 25°C, pH 8.0 Neisseria meningitidis
2.6
-
 L-methionine (R)-S-oxide mutant C84A/C136A, 25°C, pH 8.0 Neisseria meningitidis
8.5
-
 L-methionine (R)-S-oxide mutant C136A, 25°C, pH 8.0 Neisseria meningitidis
13
-
 L-methionine (R)-S-oxide mutant C94A/C136A, 25°C, pH 8.0 Neisseria meningitidis
14
-
 L-methionine (R)-S-oxide wild-type, 25°C, pH 8.0 Neisseria meningitidis
18
-
 L-methionine (R)-S-oxide mutant C84A, 25°C, pH 8.0 Neisseria meningitidis