Cloned (Comment) | Organism |
---|---|
expression inm Escherichia coli, recombinant proteins corresponding to MsrABTk and the individual domains (MsrATk and MsrBTk) are produced | Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
dithiothreitol | MsrB domain of MsrABTk, in the presence of 80 mM L-methionine (S)-sulfoxide | Thermococcus kodakarensis | |
67 | - |
L-methionine (R)-sulfoxide | MsrB domain of MsrABTk, in the presence of 20 mM dithiothreiol | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | presence of MsrABTk is greater in Thermococcus kodakaraensis cells grown at suboptimal temperatures (60 to 70°C) and could not be detected at 80 to 90°C. The amount of intracellular MsrABTk protein increases with exposure to higher dissolved oxygen levels, but only at suboptimal growth temperatures | Thermococcus kodakarensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dabsyl-L-methionine-(R)-S-oxide + dithiothreitol | - |
Thermococcus kodakarensis | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + dithiothreitol | the MsrB-domain of MsrABTk is strictly specific for the reduction of L-methionine (R)-sulfoxide | Thermococcus kodakarensis | L-methionine + dithiothreitol disulfide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MsrABTk | bifunctional enzyme (EC 1.8.1.11/1.8.1.12) | Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
MsrB domain of MsrABTk | Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life of MsrABTk is 30 min, half-life of MsrB domain is 31 min | Thermococcus kodakarensis |
85 | - |
75% decrease in activity after 2.5 min, MsrABTk | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | optimum pH of the MsrB domain | Thermococcus kodakarensis |