Cloned (Comment) | Organism |
---|---|
gene pilB, transposon insertion, truncated PilB enzyme forms of the enzyme lacking the MsrA domain from strain MS11, variant VD300, overexpression of the His-tagged PilB forms in Escherichia coli strain Xl-1 blue | Neisseria gonorrhoeae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of truncated PilB domain forms, which show decreased survival of the organism to reactive oxygen species, the mutations do not affect piliation, pilin production, or adherence, overview | Neisseria gonorrhoeae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
outer membrane | - |
Neisseria gonorrhoeae | 19867 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Neisseria gonorrhoeae | PilB affects the survival of the organism to reactive oxygen species, PilB is not involved in piliation, pilin production, or adherence | ? | - |
? | |
additional information | Neisseria gonorrhoeae MS11 | PilB affects the survival of the organism to reactive oxygen species, PilB is not involved in piliation, pilin production, or adherence | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria gonorrhoeae | - |
variant VD300, bifunctional enzyme MsrA/B, gene pilB | - |
Neisseria gonorrhoeae MS11 | - |
variant VD300, bifunctional enzyme MsrA/B, gene pilB | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged PilB forms in Escherichia coli strain Xl-1 blue by nickel affinity chromatography | Neisseria gonorrhoeae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PilB affects the survival of the organism to reactive oxygen species, PilB is not involved in piliation, pilin production, or adherence | Neisseria gonorrhoeae | ? | - |
? | |
additional information | the bifunctional enzyme catalyzes both reactions of MsrB or PilB, EC 1.8.4.12, and of MsrA or PilA, EC 1.8.4.11, the catalytic sites for the two different activities are localized separatly on the enzyme molecule, overview | Neisseria gonorrhoeae | ? | - |
? | |
additional information | PilB affects the survival of the organism to reactive oxygen species, PilB is not involved in piliation, pilin production, or adherence | Neisseria gonorrhoeae MS11 | ? | - |
? | |
additional information | the bifunctional enzyme catalyzes both reactions of MsrB or PilB, EC 1.8.4.12, and of MsrA or PilA, EC 1.8.4.11, the catalytic sites for the two different activities are localized separatly on the enzyme molecule, overview | Neisseria gonorrhoeae MS11 | ? | - |
? | |
N-acetyl-L-methionine-(R)-S-oxide + thioredoxin | - |
Neisseria gonorrhoeae | N-acetyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
N-acetyl-L-methionine-(R)-S-oxide + thioredoxin | - |
Neisseria gonorrhoeae MS11 | N-acetyl-L-methionine + thioredoxin disulfide + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 15000-35000, recombinant His-tagged PilB forms, SDS-PAGE | Neisseria gonorrhoeae |
Synonyms | Comment | Organism |
---|---|---|
MsrB | - |
Neisseria gonorrhoeae |
PilB | - |
Neisseria gonorrhoeae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Neisseria gonorrhoeae |