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Literature summary for 1.8.4.12 extracted from

  • Vieira Dos Santos, C.; Cuine, S.; Rouhier, N.; Rey, P.
    The Arabidopsis plastidic methionine sulfoxide reductase B proteins. Sequence and activity characteristics, comparison of the expression with plastidic methionine sulfoxide reductase A, and induction by photooxidative stress (2005), Plant Physiol., 138, 909-922.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.4.12

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis of plastidic isozymes MsrB1 and MsrB2, functional expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
 L-methionine-(R)-S-oxide recombinant isozyme MsrB2, with dithioerythritol Arabidopsis thaliana
0.079
-
 L-methionine-(R)-S-oxide recombinant isozyme MsrB1, with dithioerythritol Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast plastidic isozymes MsrB1 and MsrB2, analysis of subcellular localization Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine-(R)-S-oxide + thioredoxin Arabidopsis thaliana absolute stereospecific reduction L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 plastidic isozymes MsrB1 and MsrB2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant plastidic isozymes MsrB1 and MsrB2 from Escherichia coli Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine-(R)-S-oxide + dithioerythritol absolute stereospecific reduction, MsrB1 and MsrB2 Arabidopsis thaliana L-methionine + dithioerythritol disulfide + H2O
-
 ?
L-methionine-(R)-S-oxide + thioredoxin absolute stereospecific reduction Arabidopsis thaliana L-methionine + thioredoxin disulfide + H2O
-
 ?
L-methionine-(R)-S-oxide + thioredoxin absolute stereospecific reduction, isozyme MsrB2, no activity with isozyme MsrB1 Arabidopsis thaliana L-methionine + thioredoxin disulfide + H2O
-
 ?
additional information substrate specificity, overview, isozyme MsrB1 is not able to reduce free L-methionine-(R)-S-oxide or N-acetyl-L-methionine-(R)-S-oxide, while isozyme MsrB2 prefers protein-bound substrates such as N-acetyl-L-methionine (R)-S-oxide, overview Arabidopsis thaliana ?
-
 ?
N-acetyl-L-methionine-(R)-S-oxide + thioredoxin protein-bound substrate, preferred substrate of isozyme MsrB2 Arabidopsis thaliana N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase B
-
 Arabidopsis thaliana
MsrB
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 L-methionine-(R)-S-oxide recombinant isozyme MsrB2, with dithioerythritol Arabidopsis thaliana
0.07
-
 L-methionine-(R)-S-oxide recombinant isozyme MsrB1, with dithioerythritol Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
dithioerythritol DTE Arabidopsis thaliana
thioredoxin isozyme MsrB2, no activity with isozyme MsrB1 Arabidopsis thaliana