Cloned (Comment) | Organism |
---|---|
msr gene, DNA sequence determination and analysis, subcloning in Escherichia coli strain DH5-alpha, functional complementation of the enzyme-deficient mutant with the wild-type gene | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an enzyme-deficient mutant strain which shows diminished growth in presence of chemical oxidants with rapid loss of viability compared to the wild-type strain, activity can be recovered by complementation with the wild-type gene, study of oxidative stress resistance and colonization activity | Helicobacter pylori |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Helicobacter pylori | 16020 | - |
additional information | no activity in the cytoplasm | Helicobacter pylori | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, Msr, SDS-PAGE | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (R)-sulfoxide + thioredoxin | Helicobacter pylori | important antioxidant enzyme and colonization factor in the gastric pathogen, a methionine repair enzyme responsible for stress resistance | L-methionine + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
enzyme forms MsrA and MsrB are fused together forming a single protein termed Msr | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (R)-sulfoxide + thioredoxin | - |
Helicobacter pylori | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (R)-sulfoxide + thioredoxin | important antioxidant enzyme and colonization factor in the gastric pathogen, a methionine repair enzyme responsible for stress resistance | Helicobacter pylori | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | the enzyme also exhibits MsrA activity utilizing L-methionine (S)-sulfoxide as substrate | Helicobacter pylori | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, Msr, SDS-PAGE | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
methionine sulphoxide reductase | - |
Helicobacter pylori |
MSR | - |
Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Helicobacter pylori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Helicobacter pylori |