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Literature summary for 1.8.4.11 extracted from
- Expression and biological properties of a novel methionine sulfoxide reductase A in tobacco (Nicotiana tabacum) (2013), Protein J., 32, 266-274.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain BL21 (DE3) | Nicotiana tabacum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0404 | - |
thioredoxin | in 20 mM Tris-HCl, pH 8.0, 30 mM KCl, 10 mM MgCl2, at 37°C | Nicotiana tabacum |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Nicotiana tabacum | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20900 | - |
x * 20900, calculated from amino acid sequence | Nicotiana tabacum |
| 21000 | - |
x * 21000, SDS-PAGE | Nicotiana tabacum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dabsyl-L-methionine (S)-sulfoxide + thioredoxin | Nicotiana tabacum | - |
dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | - |
cultivar Hacana SRI | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+ affinity resin column chromatography | Nicotiana tabacum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Nicotiana tabacum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dabsyl-L-methionine (S)-sulfoxide + dithiothreitol | - |
Nicotiana tabacum | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
| dabsyl-L-methionine (S)-sulfoxide + thioredoxin | - |
Nicotiana tabacum | dabsyl-L-methionine + thioredoxin disulfide + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 21000, SDS-PAGE | Nicotiana tabacum |
| ? | x * 20900, calculated from amino acid sequence | Nicotiana tabacum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methionine sulfoxide reductase | - |
Nicotiana tabacum |
| MSRA4 | - |
Nicotiana tabacum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
the activity declines sharply after 42°C, falling to the minimum at 57°C (17.6% of maximal activity) | Nicotiana tabacum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 47 | the enzyme retains over 77% relative activity between 37 and 47°C | Nicotiana tabacum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.048 | - |
thioredoxin | in 20 mM Tris-HCl, pH 8.0, 30 mM KCl, 10 mM MgCl2, at 37°C | Nicotiana tabacum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | activity drops dramatically below pH 8.0 or above pH 9.0 | Nicotiana tabacum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | the enzyme shows more than 50% activity between pH 7.5 and 9.5 | Nicotiana tabacum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | - |
Nicotiana tabacum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Nicotiana tabacum | calculated from amino acid sequence | - |
5.7 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Nicotiana tabacum | enzyme expression is declined gradually when the plants are exposed to high salt, H2O2 and absisic acid conditions and decrease to lower level at 12 h | down |
| Nicotiana tabacum | the expression is slightly up-regulated in a time-dependent manner after treatment with low temperature and drought within 12 h | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | overexpression of MSRA4 in Escherichia coli cells enhances resistance to H2O2 toxicity | Nicotiana tabacum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.197 | - |
thioredoxin | in 20 mM Tris-HCl, pH 8.0, 30 mM KCl, 10 mM MgCl2, at 37°C | Nicotiana tabacum | |
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