Literature summary for 1.8.4.11 extracted from

Tete-Favier, F.; Cobessi, D.; Leonard, G.A.; Azza, S.; Talfournier, F.; Boschi-Muller, S.; Branlant, G.; Aubry, A.
Crystallization and preliminary X-ray diffraction studies of the peptide methionine sulfoxide reductase from Escherichia coli (2000), Acta Crystallogr. Sect. D, 56, 1194-1197.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene msrA, expression in strain B834(DE3)	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
15-50 mg/ml purified recombinant MsrA in 50 mM Tris-HCl, pH 8.0, 2 mM EDTA, and 10 mM DTT, hanging drop vapour diffusion method, droplet size is 0.004-0.008 ml, equal volumes of protein and precipitant solution, X-ray diffraction structure determination and analysis at 1.9 A resolution	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
peptide-L-methionine-(S)-S-oxide + thioredoxin	Escherichia coli	MsrA is involved in the antioxidant defense	peptide-L-methionine + thioredoxin disulfide + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant MsrA from strain B834(DE3)	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
peptide-L-methionine-(S)-S-oxide + DTT	-	Escherichia coli	peptide-L-methionine + DTT disulfide + H2O	-	?
peptide-L-methionine-(S)-S-oxide + thioredoxin	-	Escherichia coli	peptide-L-methionine + thioredoxin disulfide + H2O	-	?
peptide-L-methionine-(S)-S-oxide + thioredoxin	MsrA is involved in the antioxidant defense	Escherichia coli	peptide-L-methionine + thioredoxin disulfide + H2O	-	?

Synonyms

Synonyms	Comment	Organism
MsrA	-	Escherichia coli
peptide methionine sulfoxide reductase	-	Escherichia coli
PMSR	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
dithiothreitol	-	Escherichia coli
thioredoxin	-	Escherichia coli

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Release 2023.1 (January 2023)