Literature summary for 1.8.4.11 extracted from

Lowther, W.T.; Weissbach, H.; Etienne, F.; Brot, N.; Matthews, B.W.
The mirrored methionine sulfoxide reductases of Neisseria gonorrhoeae pilB (2002), Nat. Struct. Biol., 9, 348-352.

Search for more literature for 1.8.4.11

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of the full length tandem enzyme, the MsrA, and the MsrB domains, all His-tagged, in Escherichia coli	Neisseria gonorrhoeae

Organism

Organism	UniProt	Comment	Textmining
Neisseria gonorrhoeae	P14930	posesses MsrA and MsrB domains; PilB enzyme has 2 catalytic domains showing MsrA, methionine S-oxide reductase (S-form oxidizing), and MsrB, methionine S-oxide reductase (R-form oxidizing), activity, respectively	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged full length tandem enzyme, MsrA, and MsrB domains from Escherichia coli, tags are removed by thrombin digestion	Neisseria gonorrhoeae

Reaction

Reaction	Comment	Organism	Reaction ID
L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O	reaction mechanism, modeling of substrate binding at the active site, Cys72 is involved	Neisseria gonorrhoeae	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.083	-	purified recombinant MsrA/MsrB tandem domain, substrate L-methionine (S)-sulfoxide	Neisseria gonorrhoeae
0.15	-	purified recombinant MsrA domain alone, substrate L-methionine (S)-sulfoxide	Neisseria gonorrhoeae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-methionine (S)-sulfoxide + thioredoxin	MsrA activity of the tandem domains of PilB, the MsrA domain alone does very poorly utilize the R-isomer	Neisseria gonorrhoeae	L-methionine + thioredoxin disulfide + H2O	-	?
additional information	the tandem domains of PilB also posesses MsrB activity utilizing L-methionine (R)-sulfoxide as substrate, the MsrB domain alone does not utilize the S-isomer	Neisseria gonorrhoeae	?	-	?

Subunits

Subunits	Comment	Organism
More	enzyme domain and active site structure, MsrA domain comprises residues 181-362, overview	Neisseria gonorrhoeae

Synonyms

Synonyms	Comment	Organism
methionine sulfoxide reductase	-	Neisseria gonorrhoeae
MsrA	-	Neisseria gonorrhoeae
PilB	-	Neisseria gonorrhoeae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Neisseria gonorrhoeae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.9	-	assay at	Neisseria gonorrhoeae

Cofactor

Cofactor	Comment	Organism	Structure
thioredoxin	-	Neisseria gonorrhoeae

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Release 2023.1 (January 2023)