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Literature summary for 1.8.4.11 extracted from

  • Singh, V.K.; Moskovitz, J.
    Multiple methionine sulfoxide reductase genes in Staphylococcus aureus: expression of activity and roles in tolerance of oxidative stress (2003), Microbiology, 149, 2739-2747.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
complementation of the msrA1/msrA2 double mutant with the msr1 construct, promotor exchange between msrA1 and msrA2 Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
additional information construction of a msrA2 knockout mutant, a msrA1 knockout mutant, and a msrA1/msrA2 double knockout mutant, the msrA2 mutant strain and the msrA1/msrA2 double mutant strain show H2O2 tolerance like the wild-type parent strain, but the double mutant strain complemented by msrA2 is H2O2 susceptible, effects of H2O2 or oxidative stress are related to the promotors, overview Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-methionine (S)-sulfoxide + thioredoxin Staphylococcus aureus MsrAs are specific for the (S)-form of the substrate L-methionine + thioredoxin disulfide + H2O
-
?
additional information Staphylococcus aureus the MsrA1/MsrB system is physiologically more significant in Staphylococcus aureus than MsrA2 ?
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
strains RN450 and BB270, and derivatives, 3 genes msrA1, msrA2, and msrA3 encoding MsrA enzyme forms
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
69% of wild-type strain RB450 MsrA activity belongs to MsrA1, 8% to MsrA2, and 23% to MsrA3 Staphylococcus aureus
0.00006
-
msrA1/msrA2 double knockout strain RN450 Staphylococcus aureus
0.00006
-
msrA1/msrA2 double knockout strain RN450, substrate L-methionine (S)-sulfoxide Staphylococcus aureus
0.00008
-
msrA1 knockout strain RN450 Staphylococcus aureus
0.00008
-
msrA1 knockout strain RN450, substrate L-methionine (S)-sulfoxide Staphylococcus aureus
0.00024
-
msrA2 knockout strain RN450 Staphylococcus aureus
0.00024
-
msrA2 knockout strain RN450, substrate L-methionine (S)-sulfoxide Staphylococcus aureus
0.00026
-
wild-type strain RN450 Staphylococcus aureus
0.00026
-
wild-type strain RN450, substrate L-methionine (S)-sulfoxide Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine (S)-sulfoxide + thioredoxin MsrAs are specific for the (S)-form of the substrate Staphylococcus aureus L-methionine + thioredoxin disulfide + H2O
-
?
additional information the MsrA1/MsrB system is physiologically more significant in Staphylococcus aureus than MsrA2 Staphylococcus aureus ?
-
?

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase
-
Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
Staphylococcus aureus