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Literature summary for 1.8.4.11 extracted from

  • Spector, D.; Etienne, F.; Brot, N.; Weissbach, H.
    New membrane-associated and soluble peptide methionine sulfoxide reductases in Escherichia coli (2003), Biochem. Biophys. Res. Commun., 302, 284-289.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information construction of a MsrA/MsrB double mutant Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-associated isozyme, MsrA Escherichia coli 16020
-
soluble soluble isozyme MsrA1 Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein-L-methionine (S)-sulfoxide + thioredoxin Escherichia coli MsrA and the soluble isozyme MsrA1 are specific for the S-form, the membrane-associated isozyme reduces both R- and S-stereoisomers of methionine sulfoxide, N-acetylmethionine sulfoxide, and D-Ala-Met-enkephalin protein-L-methionine + thioredoxin disulfide + H2O
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
wild-type strain MC1061, isozyme MsrA, a membrane-associated isozyme, and a soluble isozyme MsrA1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0004
-
membrane vesicles, substrate N-acetyl-L-methionine-(R)-sulfoxide Escherichia coli
0.00044
-
MsrA, substrate N-acetyl-L-methionine-(S)-sulfoxide Escherichia coli
0.00047
-
membrane vesicles, substrate N-acetyl-L-methionine-(S)-sulfoxide Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine (R)-sulfoxide + thioredoxin the membrane-associated isozyme reduces both R- and S-stereoisomers of methionine sulfoxide in proteins Escherichia coli L-methionine + thioredoxin disulfide + H2O
-
?
L-methionine (S)-sulfoxide + thioredoxin MsrA and soluble isozyme MsrA1 are specific for the S-form, the membrane-associated isozyme reduces both R- and S-stereoisomers of methionine sulfoxide in proteins Escherichia coli L-methionine + thioredoxin disulfide + H2O
-
?
additional information the enzymes utilize free and protein-bound L-methionine and N-acetyl-L-methionine as substrates Escherichia coli ?
-
?
N-acetyl-L-methionine (R)-sulfoxide + thioredoxin the membrane-associated isozyme reduces both R- and S-stereoisomer of methionine sulfoxide in proteins Escherichia coli N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
?
N-acetyl-L-methionine (S)-sulfoxide + thioredoxin MsrA and soluble isozyme MsrA1 are specific for the S-form, the membrane-associated isozyme reduces both R- and S-stereoisomers Escherichia coli N-acetyl-L-methionine + thioredoxin disulfide + H2O
-
?
protein-L-methionine (S)-sulfoxide + thioredoxin MsrA and the soluble isozyme MsrA1 are specific for the S-form, the membrane-associated isozyme reduces both R- and S-stereoisomers of methionine sulfoxide, N-acetylmethionine sulfoxide, and D-Ala-Met-enkephalin Escherichia coli protein-L-methionine + thioredoxin disulfide + H2O
-
?

Synonyms

Synonyms Comment Organism
MsrA
-
Escherichia coli
peptide methionine sulfoxide reductase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
dithiothreitol MsrA can also utilize DTT as reductant, the membrane-isozyme shows only poor activity, while MsrA1 is not active with DTT Escherichia coli
thioredoxin
-
Escherichia coli