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Literature summary for 1.8.4.11 extracted from

  • Moskovitz, J.; Singh, V.K.; Requena, J.; Wilkinson, B.J.; Jayaswal, R.K.; Stadtman, E.R.
    Purification and characterization of methionine sulfoxide reductases from mouse and Staphylococcus aureus and their substrate stereospecificity (2002), Biochem. Biophys. Res. Commun., 290, 62-65.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mrsA1, and msrA2 are transcribed as polycistronic transcript, overexpression of MsrA1, and MsrA2 as His-tagged proteins in Escherichia coliBL21(DE3) Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
MsrA, which appears in 2 enzyme forms
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged MsrA1, and MsrA2 from Escherichia coli by nickel affinity chromatography Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.063
-
purified recombinant MsrA1, substrate L-methionine (S)-sulfoxide Staphylococcus aureus
0.24
-
purified recombinant MsrA2, substrate L-methionine (S)-sulfoxide Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-methionine (S)-sulfoxide + thioredoxin the 2 MsrA enzymes are absolutely specific for the S-form of the substrate Staphylococcus aureus L-methionine + thioredoxin disulfide + H2O
-
?

Synonyms

Synonyms Comment Organism
methionine sulfoxide reductase
-
Staphylococcus aureus
MsrA
-
Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
Staphylococcus aureus