Literature summary for 1.8.4.11 extracted from

Lowther, W.T.; Brot, N.; Weissbach, H.; Honek, J.F.; Matthews, B.W.
Thiol-disulfide exchange is involved in the catalytic mechanism of peptide methionine sulfoxide reductase (2000), Proc. Natl. Acad. Sci. USA, 97, 6463-6468.

Search for more literature for 1.8.4.11

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of N-terminally His-tagged wild-type and mutant MsrAs in Escherichia coli	Bos taurus

Protein Variants

Protein Variants	Comment	Organism
C107S	site-directed mutagenesis, the mutant shows 14% increased activity with DTT and 4% with thioredoxin compared to the wild-type enzyme	Bos taurus
C107S/C218S	site-directed mutagenesis, the mutant shows 78% reduced activity with DTT and 94% with thioredoxin compared to the wild-type enzyme	Bos taurus
C107S/C218S/C227S	site-directed mutagenesis, the mutant shows 61% reduced activity with DTT and 92% with thioredoxin compared to the wild-type enzyme	Bos taurus
C107S/C227S	site-directed mutagenesis, the mutant shows 4% reduced activity with DTT and 86% with thioredoxin compared to the wild-type enzyme	Bos taurus
C218S	site-directed mutagenesis, the mutant shows 65% reduced activity with DTT and 78% with thioredoxin compared to the wild-type enzyme	Bos taurus
C218S/C227S	site-directed mutagenesis, the mutant shows 58% reduced activity with DTT and 96% with thioredoxin compared to the wild-type enzyme	Bos taurus
C227S	site-directed mutagenesis, the mutant shows 11% reduced activity with DTT and 81% with thioredoxin compared to the wild-type enzyme	Bos taurus
C72S	site-directed mutagenesis, inactive mutant, no disulfide bond in the mutant enzyme	Bos taurus
C72S/C107S/C227S	site-directed mutagenesis, inactuve mutant	Bos taurus
C72S/C218S	site-directed mutagenesis, inactive mutant	Bos taurus
additional information	free sulfhydryl content and disulfide bond numbers in wild-type and mutant enzymes, overview	Bos taurus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	the enzyme does not require meal ions for activity, free sulfhydryl content and disulfide bond numbers in wild-type and mutant enzymes, overview	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His-tagged wild-type and mutant MsrAs from Escherichia coli by nickel affinity chromatography and dialysis	Bos taurus

Reaction

Reaction	Comment	Organism	Reaction ID
peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin	catalytic mechanism, Cys72 is essential for activity forming disulfide bonds with either Cys218 or Cys227	Bos taurus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-acetyl-L-methionine-(S)-S-oxide + DTT	stereospecific reduction	Bos taurus	N-acetyl-L-methionine + DTT disulfide + H2O	-	?
N-acetyl-L-methionine-(S)-S-oxide + thioredoxin	stereospecific reduction	Bos taurus	N-acetyl-L-methionine + thioredoxin disulfide + H2O	-	?

Synonyms

Synonyms	Comment	Organism
MsrA	-	Bos taurus
peptide methionine sulfoxide reductase	-	Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Bos taurus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Bos taurus

Cofactor

Cofactor	Comment	Organism	Structure
dithiothreitol	-	Bos taurus
thioredoxin	-	Bos taurus

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Release 2023.1 (January 2023)