Cloned (Comment) | Organism |
---|---|
APR-B, DNA and amino acid sequence determination and analysis, semiquantitative expression analysis, overexpression of His-tagged APR-B isozyme in Escherichia coli | Physcomitrium patens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of APR-B knockout plants, the mutant plants are able to grow on sulfate as a sole sulfur source, and the content of low molecular weight thiols is not different from wild-type plants. However, when treated with low concentrations of cadmium, the APR-B knockout plants are more sensitive than wild-type plants, phenotype, overview | Physcomitrium patens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Physcomitrium patens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenylyl sulfate + thioredoxin | Physcomitrium patens | in Physcomitrella patens, APS reduction is not the major control step of sulfate assimilation | AMP + sulfite + thioredoxin disulfide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physcomitrium patens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenylyl sulfate + thioredoxin | - |
Physcomitrium patens | AMP + sulfite + thioredoxin disulfide | - |
? | |
adenylyl sulfate + thioredoxin | in Physcomitrella patens, APS reduction is not the major control step of sulfate assimilation | Physcomitrium patens | AMP + sulfite + thioredoxin disulfide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APR-B | - |
Physcomitrium patens |
APS reductase | - |
Physcomitrium patens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Physcomitrium patens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Physcomitrium patens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | PpAPR-B does not contain the FeS cluster, which is believed to determine the substrate specificity of other APR enzymes from seed plants | Physcomitrium patens | |
thioredoxin | - |
Physcomitrium patens |