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Literature summary for 1.8.4.10 extracted from
- Identification of a new class of 5'-adenylylsulfate (APS) reductases from sulfate-assimilating bacteria (2000), J. Bacteriol., 182, 135-142.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not activated by sodium sulfate | Pseudomonas aeruginosa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-AMP | competitive vs. adenosine-5'-phosphosulfate | Pseudomonas aeruginosa |  |
| adenosine-5'-phosphosulfate | - |
Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00175 | - |
adenosine-5'-phosphosulfate | - |
Pseudomonas aeruginosa | |
| 0.0196 | - |
thioredoxin | - |
Pseudomonas aeruginosa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-adenylyl sulfate + thioredoxin | Pseudomonas aeruginosa | no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | Pseudomonas putida | involved in assimilatory sulfate reduction | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | Mycobacterium sp. | involved in assimilatory sulfate reduction | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | Ralstonia pickettii | involved in assimilatory sulfate reduction | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | Rhizobium tropici | involved in assimilatory sulfate reduction | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | Burkholderia cepacia | involved in assimilatory sulfate reduction | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | Pseudomonas aeruginosa | involved in assimilatory sulfate reduction | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | Burkholderia cepacia DBO1 / ATCC 29424 | involved in assimilatory sulfate reduction | AMP + sulfite + thioredoxin disulfide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | Q9RFS6 | - |
- |
| Burkholderia cepacia DBO1 / ATCC 29424 | Q9RFS6 | - |
- |
| Mycobacterium sp. | - |
- |
- |
| Pseudomonas aeruginosa | O05927 | - |
- |
| Pseudomonas putida | - |
- |
- |
| Ralstonia pickettii | - |
- |
- |
| Rhizobium tropici | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-adenylyl sulfate + thioredoxin | - |
Pseudomonas putida | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | - |
Mycobacterium sp. | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | - |
Ralstonia pickettii | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | - |
Rhizobium tropici | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | - |
Burkholderia cepacia | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS | Pseudomonas aeruginosa | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | involved in assimilatory sulfate reduction | Pseudomonas putida | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | involved in assimilatory sulfate reduction | Mycobacterium sp. | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | involved in assimilatory sulfate reduction | Ralstonia pickettii | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | involved in assimilatory sulfate reduction | Rhizobium tropici | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | involved in assimilatory sulfate reduction | Burkholderia cepacia | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | involved in assimilatory sulfate reduction | Pseudomonas aeruginosa | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | - |
Burkholderia cepacia DBO1 / ATCC 29424 | AMP + sulfite + thioredoxin disulfide | - |
? | |
| 5'-adenylyl sulfate + thioredoxin | involved in assimilatory sulfate reduction | Burkholderia cepacia DBO1 / ATCC 29424 | AMP + sulfite + thioredoxin disulfide | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | - |
Pseudomonas putida | |
| thioredoxin | - |
Mycobacterium sp. | |
| thioredoxin | - |
Ralstonia pickettii | |
| thioredoxin | - |
Rhizobium tropici | |
| thioredoxin | - |
Burkholderia cepacia | |
| thioredoxin | 5fold lower activity with dithiothreithol, 70fold lower activity with E. coli Grx1 or dithionite, 580fold lower activity with lipoic acid, no activity with ferredoxin | Pseudomonas aeruginosa | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
adenosine-5'-phosphosulfate | - |
Pseudomonas aeruginosa | |
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