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Literature summary for 1.8.4.1 extracted from
- Characterization of glutathione-homocystine transhydrogenase as a novel isoform of glutathione S-transferase from Aspergillus flavipes (2015), Pharm. Chem. J., 49, 373-383 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | maximum activity is obtained by nutritional optimization using GSH (0.4%) combined with homocystine (0.01%) and glucose (0.4%), NADH (30 mM) at medium initial pH 7.8. The yield of GHTHase is increased about 1.2 times upon starvation of the culture for two days, as compared to the non-sulfur starved culture. The GHTHase activity is increased by 13.7fold | Aspergillus flavipes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14.3 | - |
glutathione | pH 5.2, 40°C | Aspergillus flavipes |  |
| 15.1 | - |
cysteine | pH 5.2, 40°C | Aspergillus flavipes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
PAGE | Aspergillus flavipes |
| 79000 | 80000 | gel filtration | Aspergillus flavipes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus flavipes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus flavipes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 289.9 | - |
pH 5.2, 40°C | Aspergillus flavipes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 glutathione + homocystine | - |
Aspergillus flavipes | glutathione disulfide + 2 homocysteine | - |
? | |
| 2 lysine + homocystine | - |
Aspergillus flavipes | glutathione disulfide + 2 homocysteine | - |
? | |
| cysteine + homocystine | - |
Aspergillus flavipes | cystine + 2 homocysteine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, SDS-PAGE | Aspergillus flavipes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Aspergillus flavipes |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.056 | - |
cysteine | pH 5.2, 40°C | Aspergillus flavipes | |
| 0.078 | - |
glutathione | pH 5.2, 40°C | Aspergillus flavipes | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.6 | - |
Aspergillus flavipes |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Aspergillus flavipes |
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