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Literature summary for 1.8.3.7 extracted from

  • Carlson, B.L.; Ballister, E.R.; Skordalakes, E.; King, D.S.; Breidenbach, M.A.; Gilmore, S.A.; Berger, J.M.; Bertozzi, C.R.
    Function and structure of a prokaryotic formylglycine-generating enzyme (2008), J. Biol. Chem., 283, 20117-20125 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.3.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis
expressed in Escherichia coli BL21(DE3) cells Streptomyces coelicolor

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method, using 100 mM Tris, 2.4 M ammonium formate, 0.3% (w/v) beta-octylglucoside, 3.2% (v/v) 2-butanol, pH 8.0 Streptomyces coelicolor

Protein Variants

Protein Variants Comment Organism
C263S inactive Mycobacterium tuberculosis
C263S inactive Streptomyces coelicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the enzyme requires a Ca2+ ion Streptomyces coelicolor
additional information the enzyme does not require a divalent cation Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-
Streptomyces coelicolor
-
-
-
Streptomyces coelicolor ATCC BAA-471
-
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography Streptomyces coelicolor
HisTrap column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
LCSPSRGSLFTGR + O2 + dithiothreitol
-
 Streptomyces coelicolor leucyl-formylglycyl-SPSRGSLFTGR + ?
-
 ?
LCSPSRGSLFTGR + O2 + dithiothreitol
-
 Streptomyces coelicolor ATCC BAA-471 leucyl-formylglycyl-SPSRGSLFTGR + ?
-
 ?
LCSPSRGSLFTGR + O2 + dithiothreitol
-
 Mycobacterium tuberculosis leucyl-formylglycyl-SPSRGSLFTGR + sulfide + oxidized dithiothreitol + H2O
-
 ?
LCSPSRGSLFTGR + O2 + dithiothreitol
-
 Mycobacterium tuberculosis H37Rv leucyl-formylglycyl-SPSRGSLFTGR + sulfide + oxidized dithiothreitol + H2O
-
 ?

Synonyms

Synonyms Comment Organism
FGE
-
 Mycobacterium tuberculosis
FGE
-
 Streptomyces coelicolor