Protein Variants | Comment | Organism |
---|---|---|
C187A/C231A/C284S/C298A | the mutant shows increased activity compared to the wild type enzyme | Thermomonospora curvata |
C274S | inactive | Thermomonospora curvata |
C296S | inactive | Thermomonospora curvata |
S266A | the mutant shows reduced activity compared to the wild type enzyme | Thermomonospora curvata |
S290K | the mutant shows very low activity compared to the wild type enzyme | Thermomonospora curvata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant C187A/C231A/C284S/C298A, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
0.52 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant S266A, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
0.58 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | wild type enzyme, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu+ | required | Thermomonospora curvata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomonospora curvata | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + O2 + hydrogen sulfide + oxidized dithiothreitol | - |
Thermomonospora curvata | ? | - |
? | |
additional information | low activity with Abz-SALSSPTRA-NH2 | Thermomonospora curvata | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FGE | - |
Thermomonospora curvata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant S266A, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
0.027 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | wild type enzyme, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
0.07 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant C187A/C231A/C284S/C298A, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00011 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant S290K, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
0.00082 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant S266A, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
0.048 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | wild type enzyme, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata | |
0.333 | - |
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 | mutant C187A/C231A/C284S/C298A, at pH 8.0, temperature not specified in the publication | Thermomonospora curvata |