Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteamine | - |
Thermomonospora curvata | |
dithiothreitol | - |
Thermomonospora curvata |
Cloned (Comment) | Organism |
---|---|
- |
Thermomonospora curvata |
Protein Variants | Comment | Organism |
---|---|---|
C187A/C231A/C284S/C298A | increased activity | Thermomonospora curvata |
C269S | almost no activity | Thermomonospora curvata |
C274S | almost no activity | Thermomonospora curvata |
S266A | reduced activity | Thermomonospora curvata |
S290K | reduced activity | Thermomonospora curvata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
Abz-SALCSPTRA-NH2 | mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 | Thermomonospora curvata | |
0.52 | - |
Abz-SALCSPTRA-NH2 | mutant enzyme S266A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 | Thermomonospora curvata | |
0.58 | - |
Abz-SALCSPTRA-NH2 | wild-type enzyme, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 | Thermomonospora curvata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu+ | copper-metalloenzyme, one equivalent of Cu+ per acive site. No other transition metals can replace copper | Thermomonospora curvata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomonospora curvata | Q9F3C7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-SALCSPTRA-NH2 + O2 + reduced dithiothreitol | - |
Thermomonospora curvata | Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FGE | - |
Thermomonospora curvata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
Abz-SALCSPTRA-NH2 | mutant enzyme S266A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 | Thermomonospora curvata | |
0.027 | - |
Abz-SALCSPTRA-NH2 | wild-type enzyme, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 | Thermomonospora curvata | |
0.07 | - |
Abz-SALCSPTRA-NH2 | mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 | Thermomonospora curvata |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0101 | - |
Abz-SALCSPTRA-NH2 | mutant enzyme S290K, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 | Thermomonospora curvata | |
0.333 | - |
Abz-SALCSPTRA-NH2 | mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 | Thermomonospora curvata | |
8.17 | - |
Abz-SALCSPTRA-NH2 | mutant enzyme S266A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 | Thermomonospora curvata | |
48.33 | - |
Abz-SALCSPTRA-NH2 | wild-type enzyme, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 | Thermomonospora curvata |