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Literature summary for 1.8.3.7 extracted from

  • Knop M.; Dang, T.Q.; Jeschke, G.; Seebeck, F.P.
    Copper is a Cofactor of the Formylglycine-Generating Enzyme (2017), ChemBioChem, 18, 161-165.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.3.7

Activating Compound

Activating Compound Comment Organism Structure
cysteamine
-
 Thermomonospora curvata
dithiothreitol
-
 Thermomonospora curvata

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermomonospora curvata

Protein Variants

Protein Variants Comment Organism
C187A/C231A/C284S/C298A increased activity Thermomonospora curvata
C269S almost no activity Thermomonospora curvata
C274S almost no activity Thermomonospora curvata
S266A reduced activity Thermomonospora curvata
S290K reduced activity Thermomonospora curvata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 Abz-SALCSPTRA-NH2 mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 Thermomonospora curvata
0.52
-
 Abz-SALCSPTRA-NH2 mutant enzyme S266A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 Thermomonospora curvata
0.58
-
 Abz-SALCSPTRA-NH2 wild-type enzyme, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 Thermomonospora curvata

Metals/Ions

Metals/Ions Comment Organism Structure
Cu+ copper-metalloenzyme, one equivalent of Cu+ per acive site. No other transition metals can replace copper Thermomonospora curvata

Organism

Organism UniProt Comment Textmining
Thermomonospora curvata Q9F3C7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-SALCSPTRA-NH2 + O2 + reduced dithiothreitol
-
 Thermomonospora curvata Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol
-
 ?

Synonyms

Synonyms Comment Organism
FGE
-
 Thermomonospora curvata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 Abz-SALCSPTRA-NH2 mutant enzyme S266A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 Thermomonospora curvata
0.027
-
 Abz-SALCSPTRA-NH2 wild-type enzyme, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 Thermomonospora curvata
0.07
-
 Abz-SALCSPTRA-NH2 mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 Thermomonospora curvata

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0101
-
 Abz-SALCSPTRA-NH2 mutant enzyme S290K, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 Thermomonospora curvata
0.333
-
 Abz-SALCSPTRA-NH2 mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 Thermomonospora curvata
8.17
-
 Abz-SALCSPTRA-NH2 mutant enzyme S266A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 Thermomonospora curvata
48.33
-
 Abz-SALCSPTRA-NH2 wild-type enzyme, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.005 mM CuSO4, pH 8.0 Thermomonospora curvata