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Literature summary for 1.8.3.7 extracted from

  • Knop, M.; Engi, P.; Lemnaru, R.; Seebeck, F.P.
    In Vitro Reconstitution of Formylglycine-Generating Enzymes Requires Copper(I) (2015), ChemBioChem, 16, 2147-2150.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Mycolicibacterium smegmatis
-
Thermomonospora curvata

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Thermomonospora curvata

Protein Variants

Protein Variants Comment Organism
C187A/C231A/C284S/C298A ten-fold increased activity compared to wild-type Thermomonospora curvata

Metals/Ions

Metals/Ions Comment Organism Structure
Cu+ CuSO4 and CuCl are almost equally efficient Mycolicibacterium smegmatis
Cu+ CuSO4 and CuCl are almost equally efficient Thermomonospora curvata
Cu2+ CuSO4 and CuCl are almost equally efficient Mycolicibacterium smegmatis
Cu2+ CuSO4 and CuCl are almost equally efficient Thermomonospora curvata

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-
Mycolicibacterium smegmatis DSM 43465
-
-
-
Thermomonospora curvata
-
-
-
Thermomonospora curvata DSM 43183
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-SALCSPTRA-NH2 + O2 + reduced dithiothreitol
-
Mycolicibacterium smegmatis Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol
-
?
Abz-SALCSPTRA-NH2 + O2 + reduced dithiothreitol
-
Thermomonospora curvata Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol
-
?
Abz-SALCSPTRA-NH2 + O2 + reduced dithiothreitol
-
Mycolicibacterium smegmatis DSM 43465 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol
-
?
Abz-SALCSPTRA-NH2 + O2 + reduced dithiothreitol
-
Thermomonospora curvata DSM 43183 Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol
-
?

Synonyms

Synonyms Comment Organism
FGE
-
Mycolicibacterium smegmatis
FGE
-
Thermomonospora curvata

Temperature Stability [掳C]

Temperature Stability Minimum [掳C] Temperature Stability Maximum [掳C] Comment Organism
43
-
mutant enzyme C187A/C231A/C284S/C298A, stable up to Thermomonospora curvata
51
-
wild-type enzyme, stable up to Thermomonospora curvata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
Abz-SALCSPTRA-NH2 mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, pH 8.0 Thermomonospora curvata
0.027
-
Abz-SALCSPTRA-NH2 wild-type enzyme, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.002 mM CuSO4, pH 8.0 Thermomonospora curvata
0.07
-
Abz-SALCSPTRA-NH2 mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.002 mM CuSO4, pH 8.0 Thermomonospora curvata

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7
-
Abz-SALCSPTRA-NH2 wild-type enzyme, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, pH 8.0 Thermomonospora curvata
17
-
Abz-SALCSPTRA-NH2 mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, pH 8.0 Thermomonospora curvata
48.3
-
Abz-SALCSPTRA-NH2 wild-type enzyme, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.002 mM CuSO4, pH 8.0 Thermomonospora curvata
333.3
-
Abz-SALCSPTRA-NH2 mutant enzyme C187A/C231A/C284S/C298A, 25掳C, 5 mM DTT, 50 mM EDTA, 50 mM NaCl, and 50 mM Tris路HCl, 0.002 mM CuSO4, pH 8.0 Thermomonospora curvata