Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Gallus gallus |
Protein Variants | Comment | Organism |
---|---|---|
R472M | introduction of predicted catalytic site residues of assimilatory nitrate reductase, kinetic analysis | Homo sapiens |
R472Q | introduction of predicted catalytic site residues of assimilatory nitrate reductase, kinetic analysis | Homo sapiens |
V474M | active site mutant, kinetic analysis | Homo sapiens |
Y322F/R450M | introduction of predicted catalytic site residues of assimilatory nitrate reductase, markedly decreased ability to bind sulfite at pH 8.5 | Gallus gallus |
Y343F/R472Q | active site mutant, kinetic analysis | Homo sapiens |
Y343N | active site mutant, kinetic analysis | Homo sapiens |
Y343N/R472M | active site mutant, kinetic analysis | Homo sapiens |
Y343N/R472M/V474M | active site mutant, kinetic analysis | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00046 | - |
sulfite | mutant V474M, 25°C, pH 6.0 | Homo sapiens | |
0.0013 | - |
sulfite | wild-type, 25°C, pH 6.0 | Homo sapiens | |
0.00133 | - |
sulfite | wild-type, 25°C, pH 7.0 | Gallus gallus | |
0.00134 | - |
sulfite | mutant V474M, 25°C, pH 7.0 | Homo sapiens | |
0.0019 | - |
sulfite | mutant R472M, 25°C, pH 6.0 | Homo sapiens | |
0.0023 | - |
sulfite | mutant R472Q, 25°C, pH 6.0 | Homo sapiens | |
0.0025 | - |
sulfite | mutant R472M, 25°C, pH 7.0 | Homo sapiens | |
0.0027 | - |
sulfite | wild-type, 25°C, pH 7.0 | Homo sapiens | |
0.0032 | - |
sulfite | mutant V474M, 25°C, pH 8.0 | Homo sapiens | |
0.0035 | - |
sulfite | mutant R472Q, 25°C, pH 7.0 | Homo sapiens | |
0.0043 | - |
sulfite | wild-type, 25°C, pH 8.0 | Homo sapiens | |
0.0047 | - |
sulfite | mutant R472M, 25°C, pH 8.0 | Homo sapiens | |
0.008 | - |
sulfite | wild-type, 25°C, pH 8.5 | Gallus gallus | |
0.0083 | - |
sulfite | wild-type, 25°C, pH 8.5 | Homo sapiens | |
0.013 | - |
sulfite | mutant R472Q, 25°C, pH 8.0 | Homo sapiens | |
0.0144 | - |
sulfite | mutant V474M, 25°C, pH 9.0 | Homo sapiens | |
0.016 | - |
sulfite | mutant R472Q, 25°C, pH 8.5 | Homo sapiens | |
0.021 | - |
sulfite | mutant R472M, 25°C, pH 9.0 | Homo sapiens | |
0.022 | - |
sulfite | wild-type, 25°C, pH 9.0 | Homo sapiens | |
0.0319 | - |
sulfite | mutant V474M, 25°C, pH 9.5 | Homo sapiens | |
0.0354 | - |
sulfite | mutant V474M, 25°C, pH 8.5 | Homo sapiens | |
0.04 | - |
sulfite | mutant V474M, 25°C, pH 10.0 | Homo sapiens | |
0.045 | - |
sulfite | mutant R472Q, 25°C, pH 9.0 | Homo sapiens | |
0.0529 | - |
sulfite | wild-type, 25°C, pH 10.0 | Homo sapiens | |
0.0537 | - |
sulfite | mutant R472M, 25°C, pH 9.5 | Homo sapiens | |
0.0614 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 6.0 | Homo sapiens | |
0.067 | - |
sulfite | wild-type, 25°C, pH 9.5 | Homo sapiens | |
0.0877 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 7.0 | Homo sapiens | |
0.094 | - |
sulfite | mutant R472M, 25°C, pH 8.5 | Homo sapiens | |
0.0947 | - |
sulfite | mutant Y343N, 25°C, pH 7.0 | Homo sapiens | |
0.0953 | - |
sulfite | mutant Y343N, 25°C, pH 6.0 | Homo sapiens | |
0.0967 | - |
sulfite | mutant R472Q, 25°C, pH 9.5 | Homo sapiens | |
0.0969 | - |
sulfite | mutant R472M, 25°C, pH 10.0 | Homo sapiens | |
0.181 | - |
sulfite | mutant R472Q, 25°C, pH 10.0 | Homo sapiens | |
0.2827 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 8.0 | Homo sapiens | |
0.297 | - |
sulfite | mutant Y343N, 25°C, pH 8.0 | Homo sapiens | |
0.712 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 8.5 | Homo sapiens | |
0.85 | - |
sulfite | mutant Y343N, 25°C, pH 8.5 | Homo sapiens | |
1.1 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 6.0 | Homo sapiens | |
1.42 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 7.0 | Homo sapiens | |
2.14 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 8.0 | Homo sapiens | |
2.46 | - |
sulfite | mutant Y343N, 25°C, pH 9.0 | Homo sapiens | |
3.34 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 9.0 | Homo sapiens | |
4.64 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 7.0 | Homo sapiens | |
8.6 | - |
sulfite | mutant Y322N/R450M, 25°C, pH 7.0 | Gallus gallus | |
9.37 | - |
sulfite | mutant Y343N, 25°C, pH 9.5 | Homo sapiens | |
11.73 | - |
sulfite | mutant Y322N/R450M, 25°C, pH 8.5 | Gallus gallus | |
12 | - |
sulfite | mutant Y343N, 25°C, pH 10.0 | Homo sapiens | |
14 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 8.5 | Homo sapiens | |
16.8 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 6.0 | Homo sapiens | |
19.28 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 8.0 | Homo sapiens | |
39.9 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 9.5 | Homo sapiens | |
42.99 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 8.5 | Homo sapiens | |
55.5 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 9.0 | Homo sapiens | |
59.6 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 10.0 | Homo sapiens | |
85.64 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 9.0 | Homo sapiens | |
111 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 9.5 | Homo sapiens | |
208 | - |
sulfite | mutant Y343N/R472m, 25°C, pH 9.5 | Homo sapiens | |
418 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 10.0 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | P07850 | - |
- |
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + cytochrome c | - |
Homo sapiens | sulfate + reduced cytochrome c | - |
? | |
sulfite + cytochrome c | - |
Gallus gallus | sulfate + reduced cytochrome c | - |
? | |
sulfite + ferricyanide + H2O | - |
Homo sapiens | sulfate + ferrocyanide | - |
? | |
sulfite + ferricyanide + H2O | - |
Gallus gallus | sulfate + ferrocyanide | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
sulfite | mutant R472M, 25°C, pH 10.0 | Homo sapiens | |
0.52 | - |
sulfite | mutant R472Q, 25°C, pH 10.0 | Homo sapiens | |
0.97 | - |
sulfite | mutant Y343N/R472Q, 25°C, pH 9.5 | Homo sapiens | |
1.13 | - |
sulfite | mutant Y343N/R472Q, 25°C, pH 7.0 | Homo sapiens | |
1.13 | - |
sulfite | mutant Y343N/R472Q, 25°C, pH 9.0 | Homo sapiens | |
1.33 | - |
sulfite | mutant Y343N/R472Q, 25°C, pH 8.0 | Homo sapiens | |
1.35 | - |
sulfite | mutant Y343N/R472Q, 25°C, pH 6.0 | Homo sapiens | |
1.42 | - |
sulfite | mutant Y343N/R472Q, 25°C, pH 8.5 | Homo sapiens | |
1.44 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 6.0 | Homo sapiens | |
1.7 | - |
sulfite | mutant R472Q, 25°C, pH 9.5 | Homo sapiens | |
1.73 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 7.0 | Homo sapiens | |
1.8 | - |
sulfite | mutant R472M, 25°C, pH 9.5 | Homo sapiens | |
1.9 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 6.0 | Homo sapiens | |
2.05 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 8.0 | Homo sapiens | |
2.25 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 8.5 | Homo sapiens | |
2.3 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 9.0 | Homo sapiens | |
2.52 | - |
sulfite | mutant Y322N/R450M, 25°C, pH 8.5 | Gallus gallus | |
2.8 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 10.0 | Homo sapiens | |
2.96 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 10.0 | Homo sapiens | |
3.17 | - |
sulfite | mutant Y343N, 25°C, pH 6.0 | Homo sapiens | |
3.4 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 7.0 | Homo sapiens | |
3.45 | - |
sulfite | mutant R472M, 25°C, pH 9.0 | Homo sapiens | |
3.5 | - |
sulfite | mutant R472M, 25°C, pH 8.0 | Homo sapiens | |
3.58 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 8.0 | Homo sapiens | |
3.6 | - |
sulfite | mutant R472M, 25°C, pH 7.0 | Homo sapiens | |
3.8 | - |
sulfite | mutant R472M, 25°C, pH 8.5 | Homo sapiens | |
3.9 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 8.5 | Homo sapiens | |
4.24 | - |
sulfite | mutant R472Q, 25°C, pH 9.0 | Homo sapiens | |
4.38 | - |
sulfite | mutant Y343N, 25°C, pH 10.0 | Homo sapiens | |
4.6 | - |
sulfite | mutant R472Q, 25°C, pH 8.5 | Homo sapiens | |
4.83 | - |
sulfite | mutant Y322N/R450M, 25°C, pH 7.0 | Gallus gallus | |
4.9 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 9.5 | Homo sapiens | |
5 | - |
sulfite | mutant R472M, 25°C, pH 6.0 | Homo sapiens | |
5.23 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 9.5 | Homo sapiens | |
5.6 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 9.0 | Homo sapiens | |
5.8 | - |
sulfite | mutant R472Q, 25°C, pH 7.0 | Homo sapiens | |
5.96 | - |
sulfite | mutant V474M, 25°C, pH 6.0 | Homo sapiens | |
8.1 | - |
sulfite | mutant Y343N, 25°C, pH 9.5 | Homo sapiens | |
8.2 | - |
sulfite | mutant R472Q, 25°C, pH 6.0 | Homo sapiens | |
9.3 | - |
sulfite | mutant R472Q, 25°C, pH 8.0 | Homo sapiens | |
11.4 | - |
sulfite | mutant V474M, 25°C, pH 7.0 | Homo sapiens | |
12.4 | - |
sulfite | mutant V474M, 25°C, pH 10.0 | Homo sapiens | |
12.8 | - |
sulfite | mutant Y343N, 25°C, pH 7.0 | Homo sapiens | |
13 | - |
sulfite | wild-type, 25°C, pH 10.0 | Homo sapiens | |
13.2 | - |
sulfite | wild-type, 25°C, pH 6.0 | Homo sapiens | |
13.75 | - |
sulfite | mutant Y343N, 25°C, pH 8.0 | Homo sapiens | |
15.5 | - |
sulfite | mutant Y343N, 25°C, pH 9.0 | Homo sapiens | |
15.8 | - |
sulfite | mutant V474M, 25°C, pH 8.5 | Homo sapiens | |
16.9 | - |
sulfite | mutant Y343N, 25°C, pH 8.5 | Homo sapiens | |
17.1 | - |
sulfite | mutant V474M, 25°C, pH 9.5 | Homo sapiens | |
17.8 | - |
sulfite | mutant V474M, 25°C, pH 8.0 | Homo sapiens | |
19.6 | - |
sulfite | mutant V474M, 25°C, pH 9.0 | Homo sapiens | |
24.2 | - |
sulfite | wild-type, 25°C, pH 7.0 | Homo sapiens | |
25.7 | - |
sulfite | wild-type, 25°C, pH 9.0 | Homo sapiens | |
25.9 | - |
sulfite | wild-type, 25°C, pH 8.0 | Homo sapiens | |
26.3 | - |
sulfite | wild-type, 25°C, pH 9.5 | Homo sapiens | |
26.9 | - |
sulfite | wild-type, 25°C, pH 8.5 | Homo sapiens | |
36.1 | - |
sulfite | wild-type, 25°C, pH 7.0 | Gallus gallus | |
73 | - |
sulfite | wild-type, 25°C, pH 8.5 | Gallus gallus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00047 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 9.5 | Homo sapiens | |
0.00067 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 10.0 | Homo sapiens | |
0.0013 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 9.0 | Homo sapiens | |
0.0033 | - |
sulfite | mutant Y343N/R472m, 25°C, pH 8.5 | Homo sapiens | |
0.0047 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 9.5 | Homo sapiens | |
0.0049 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 10.0 | Homo sapiens | |
0.0069 | - |
sulfite | mutant Y343N/R472m, 25°C, pH 8.0 | Homo sapiens | |
0.008 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 6.0 | Homo sapiens | |
0.01 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 9.0 | Homo sapiens | |
0.0124 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 9.5 | Homo sapiens | |
0.0244 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 7.0 | Homo sapiens | |
0.0279 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 8.5 | Homo sapiens | |
0.036 | - |
sulfite | mutant Y343N, 25°C, pH 10.0 | Homo sapiens | |
0.069 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 9.0 | Homo sapiens | |
0.0866 | - |
sulfite | mutant Y343N, 25°C, pH 9.5 | Homo sapiens | |
0.167 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 8.0 | Homo sapiens | |
0.173 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 6.0 | Homo sapiens | |
0.214 | - |
sulfite | mutant Y322N/R450M, 25°C, pH 8.5 | Gallus gallus | |
0.237 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 7.0 | Homo sapiens | |
0.316 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 8.5 | Homo sapiens | |
0.632 | - |
sulfite | mutant Y343N, 25°C, pH 9.0 | Homo sapiens | |
0.725 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 8.0 | Homo sapiens | |
1.97 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 7.0 | Homo sapiens | |
1.99 | - |
sulfite | mutant Y343N, 25°C, pH 8.5 | Homo sapiens | |
2.35 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 6.0 | Homo sapiens | |
3.33 | - |
sulfite | mutant Y343N, 25°C, pH 6.0 | Homo sapiens | |
4.63 | - |
sulfite | mutant Y343N, 25°C, pH 8.0 | Homo sapiens | |
8.6 | - |
sulfite | wild-type, 25°C, pH 7.0 | Gallus gallus | |
13.5 | - |
sulfite | mutant Y343N, 25°C, pH 7.0 | Homo sapiens | |
30.8 | - |
sulfite | mutant V474M, 25°C, pH 10.0 | Homo sapiens | |
53.6 | - |
sulfite | mutant V474M, 25°C, pH 9.5 | Homo sapiens | |
136 | - |
sulfite | mutant V474M, 25°C, pH 9.0 | Homo sapiens | |
246 | - |
sulfite | wild-type, 25°C, pH 10.0 | Homo sapiens | |
392 | - |
sulfite | wild-type, 25°C, pH 9.5 | Homo sapiens | |
446 | - |
sulfite | mutant V474M, 25°C, pH 8.5 | Homo sapiens | |
558 | - |
sulfite | mutant V474M, 25°C, pH 8.0 | Homo sapiens | |
851 | - |
sulfite | mutant V474M, 25°C, pH 7.0 | Homo sapiens | |
1160 | - |
sulfite | wild-type, 25°C, pH 9.0 | Homo sapiens | |
1300 | - |
sulfite | mutant V474M, 25°C, pH 6.0 | Homo sapiens | |
3260 | - |
sulfite | wild-type, 25°C, pH 8.5 | Homo sapiens | |
5950 | - |
sulfite | wild-type, 25°C, pH 8.0 | Homo sapiens | |
8690 | - |
sulfite | wild-type, 25°C, pH 8.5 | Gallus gallus | |
8900 | - |
sulfite | wild-type, 25°C, pH 7.0 | Homo sapiens | |
10000 | - |
sulfite | wild-type, 25°C, pH 6.0 | Homo sapiens | |
27000 | - |
sulfite | mutant Y322N/R450M, 25°C, pH 7.0 | Gallus gallus |