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Literature summary for 1.8.3.1 extracted from

  • Di Salle, A.; DErrico, G.; La Cara, F.; Cannio, R.; Rossi, M.
    A novel thermostable sulfite oxidase from Thermus thermophilus: characterization of the enzyme, gene cloning and expression in Escherichia coli (2006), Extremophiles, 10, 587-598.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA
-
Thermus thermophilus
Molybdenum the addition of molybdenum to culture media at a concentration of 2.07 mM molybdate leads to a 4fold increase in activity Thermus thermophilus
additional information molybdenum concentrations higher than 2.07 mM do not show increase of enzyme activity Thermus thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain TP1000 Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
arsenite 7.4 mM, 50% inhibition Thermus thermophilus
Ca2+
-
Thermus thermophilus
additional information inhibition at 400 mM salt concentrations; no inhibition by periodate or 80 mM sulfate Thermus thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Thermus thermophilus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 45-100 mM, 54-88% increase of activity Thermus thermophilus
Molybdenum the addition of molybdenum to culture media at a concentration of 2.07 mM molybdate leads to a 4fold increase in activity Thermus thermophilus
NH4+ 30 mM, 74% increase of activity Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
gel filtration Thermus thermophilus
39100
-
SDS-PAGE Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-
Thermus thermophilus AT62
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE 52 column chromatography, Superdex X26 gel filtration and hydroxyapatite column chromatography Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.191
-
crude cell extract Thermus thermophilus
56.67
-
after 297fold purification Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + H2O + A
-
Thermus thermophilus SO42- + AH2
-
?
sulfite + H2O + A
-
Thermus thermophilus AT62 SO42- + AH2
-
?

Synonyms

Synonyms Comment Organism
SO
-
Thermus thermophilus
sulfite oxidase
-
Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 80 The enzyme retains 100% of residual activity up to 7 h of incubation at 50°C. Half life times of the enzyme at 60, 70 and 80°C are respectively 8, 6.5 and 1.5 h. The enzyme retains 30% of activity at 25°C and 45% at 90°C. Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
sulfite
-
Thermus thermophilus
53.32
-
sulfite
-
Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
-
Thermus thermophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
9.4
-
Ca2+
-
Thermus thermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Thermus thermophilus
-
-
6.8