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Literature summary for 1.8.2.7 extracted from

  • Tikhonova, T.V.; Sorokin, D.Y.; Hagen, W.R.; Khrenova, M.G.; Muyzer, G.; Rakitina, T.V.; Shabalin, I.G.; Trofimov, A.A.; Tsallagov, S.I.; Popov, V.O.
    Trinuclear copper biocatalytic center forms an active site of thiocyanate dehydrogenase (2020), Proc. Natl. Acad. Sci. USA, 117, 5280-5290 .
    View publication on PubMed
Search for more literature for 1.8.2.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) cells Thioalkalivibrio paradoxus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.17 M ammonium acetate, 0.085 M sodium citrate, pH 5.6, 25.5% (w/v) PEG 4000, 15% (v/v) glycerol or 0.17 M ammonium sulfate, 0.085 M sodium cacodylate, pH 6.5, 25.5% (w/v) PEG 8000, 15% (v/v) glycerol Thioalkalivibrio paradoxus

Protein Variants

Protein Variants Comment Organism
E288A inactive Thioalkalivibrio paradoxus
H136A inactive Thioalkalivibrio paradoxus

Inhibitors

Inhibitors Comment Organism Structure
azide
-
 Thioalkalivibrio paradoxus
azide
-
 Thioalkalivibrio thiocyanoxidans
Cyanate mixed-type inhibition Thioalkalivibrio paradoxus
Cyanate mixed-type inhibition Thioalkalivibrio thiocyanoxidans
cyanide competitive inhibition Thioalkalivibrio paradoxus
cyanide competitive inhibition Thioalkalivibrio thiocyanoxidans
additional information nitrate, nitrite, bicarbonate, sulfate, acetate, and iodide show no inhibitory effect at 1 mM Thioalkalivibrio paradoxus
additional information nitrate, nitrite, bicarbonate, sulfate, acetate, and iodide show no inhibitory effect at 1 mM Thioalkalivibrio thiocyanoxidans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 Wurster's blue at pH 9.5 and 30°C Thioalkalivibrio paradoxus
1
-
 ferricytochrome c 550 at pH 9.5 and 30°C Thioalkalivibrio paradoxus
1.2
-
 potassium ferricyanide at pH 9.5 and 30°C Thioalkalivibrio paradoxus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ contains three copper ions Thioalkalivibrio paradoxus
Cu2+ contains three copper ions Thioalkalivibrio thiocyanoxidans

Organism

Organism UniProt Comment Textmining
Thioalkalivibrio paradoxus
-
-
-
Thioalkalivibrio paradoxus ARh1
-
-
-
Thioalkalivibrio thiocyanoxidans
-
-
-
Thioalkalivibrio thiocyanoxidans ARh2
-
-
-
Thioalkalivibrio thiocyanoxidans ARh4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography and Superdex 200 gel filtration Thioalkalivibrio paradoxus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed Thioalkalivibrio paradoxus ?
-
-
additional information low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed Thioalkalivibrio thiocyanoxidans ?
-
-
additional information low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed Thioalkalivibrio paradoxus ARh1 ?
-
-
additional information low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed Thioalkalivibrio thiocyanoxidans ARh2 ?
-
-
additional information low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed Thioalkalivibrio thiocyanoxidans ARh4 ?
-
-
thiocyanate + 2 ferricytochrome c 550 + H2O
-
 Thioalkalivibrio paradoxus cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
 ?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
 Thioalkalivibrio thiocyanoxidans cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
 ?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
 Thioalkalivibrio paradoxus ARh1 cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
 ?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh2 cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
 ?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh4 cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
 ?
thiocyanate + 2 potassium ferricyanide + H2O
-
 Thioalkalivibrio paradoxus cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
 ?
thiocyanate + 2 potassium ferricyanide + H2O
-
 Thioalkalivibrio thiocyanoxidans cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
 ?
thiocyanate + 2 potassium ferricyanide + H2O
-
 Thioalkalivibrio paradoxus ARh1 cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
 ?
thiocyanate + 2 potassium ferricyanide + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh2 cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
 ?
thiocyanate + 2 potassium ferricyanide + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh4 cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
 ?
thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio thiocyanoxidans cyanate + sulfur + reduced Wurster's blue + 2 H+
-
 ?
thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh2 cyanate + sulfur + reduced Wurster's blue + 2 H+
-
 ?
thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh4 cyanate + sulfur + reduced Wurster's blue + 2 H+
-
 ?
thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio paradoxus cyanate + sulfur + reduced Wurster's blue + H+
-
 ?
thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio thiocyanoxidans cyanate + sulfur + reduced Wurster's blue + H+
-
 ?
thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio paradoxus ARh1 cyanate + sulfur + reduced Wurster's blue + H+
-
 ?
thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh2 cyanate + sulfur + reduced Wurster's blue + H+
-
 ?
thiocyanate + Wurster's blue + H2O
-
 Thioalkalivibrio thiocyanoxidans ARh4 cyanate + sulfur + reduced Wurster's blue + H+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 51891, calculated from amino acid sequence Thioalkalivibrio paradoxus
homodimer 2 * 51891, calculated from amino acid sequence Thioalkalivibrio thiocyanoxidans
homodimer 2 * 52000-53000, SDS-PAGE Thioalkalivibrio paradoxus
homodimer 2 * 52000-53000, SDS-PAGE Thioalkalivibrio thiocyanoxidans

Synonyms

Synonyms Comment Organism
TcDH
-
 Thioalkalivibrio paradoxus
TcDH
-
 Thioalkalivibrio thiocyanoxidans
thiocyanate dehydrogenase
-
 Thioalkalivibrio paradoxus
thiocyanate dehydrogenase
-
 Thioalkalivibrio thiocyanoxidans
thiocyanate: cytochrome c oxidoreductase
-
 Thioalkalivibrio paradoxus
thiocyanate: cytochrome c oxidoreductase
-
 Thioalkalivibrio thiocyanoxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Thioalkalivibrio paradoxus
9.5
-
-
 Thioalkalivibrio thiocyanoxidans

pH Range

pH Minimum pH Maximum Comment Organism
8 11 more than 50% activity between pH 8.0 and 11.0 with potassium ferricyanide as electron acceptor, more than 50% activity between pH 8.5 and 10.5 with potassium ferricyanide as electron acceptor Thioalkalivibrio paradoxus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0014
-
 cyanide at pH 9.5 and 30°C Thioalkalivibrio paradoxus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
60
-
 at pH 9.5 and 30°C Thioalkalivibrio paradoxus azide