BRENDA - Enzyme Database
show all sequences of 1.8.2.7

Trinuclear copper biocatalytic center forms an active site of thiocyanate dehydrogenase

Tikhonova, T.V.; Sorokin, D.Y.; Hagen, W.R.; Khrenova, M.G.; Muyzer, G.; Rakitina, T.V.; Shabalin, I.G.; Trofimov, A.A.; Tsallagov, S.I.; Popov, V.O.; Proc. Natl. Acad. Sci. USA 117, 5280-5290 (2020)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21 Star (DE3) cells
Thioalkalivibrio paradoxus
Crystallization (Commentary)
Crystallization (Commentary)
Organism
hanging drop vapor diffusion method, using 0.17 M ammonium acetate, 0.085 M sodium citrate, pH 5.6, 25.5% (w/v) PEG 4000, 15% (v/v) glycerol or 0.17 M ammonium sulfate, 0.085 M sodium cacodylate, pH 6.5, 25.5% (w/v) PEG 8000, 15% (v/v) glycerol
Thioalkalivibrio paradoxus
Engineering
Protein Variants
Commentary
Organism
E288A
inactive
Thioalkalivibrio paradoxus
H136A
inactive
Thioalkalivibrio paradoxus
Inhibitors
Inhibitors
Commentary
Organism
Structure
azide
-
Thioalkalivibrio paradoxus
azide
-
Thioalkalivibrio thiocyanoxidans
Cyanate
mixed-type inhibition
Thioalkalivibrio paradoxus
Cyanate
mixed-type inhibition
Thioalkalivibrio thiocyanoxidans
cyanide
competitive inhibition
Thioalkalivibrio paradoxus
cyanide
competitive inhibition
Thioalkalivibrio thiocyanoxidans
additional information
nitrate, nitrite, bicarbonate, sulfate, acetate, and iodide show no inhibitory effect at 1 mM
Thioalkalivibrio paradoxus
additional information
nitrate, nitrite, bicarbonate, sulfate, acetate, and iodide show no inhibitory effect at 1 mM
Thioalkalivibrio thiocyanoxidans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.8
-
Wurster's blue
at pH 9.5 and 30°C
Thioalkalivibrio paradoxus
1
-
ferricytochrome c 550
at pH 9.5 and 30°C
Thioalkalivibrio paradoxus
1.2
-
potassium ferricyanide
at pH 9.5 and 30°C
Thioalkalivibrio paradoxus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
contains three copper ions
Thioalkalivibrio paradoxus
Cu2+
contains three copper ions
Thioalkalivibrio thiocyanoxidans
Organism
Organism
UniProt
Commentary
Textmining
Thioalkalivibrio paradoxus
-
-
-
Thioalkalivibrio paradoxus ARh1
-
-
-
Thioalkalivibrio thiocyanoxidans
-
-
-
Thioalkalivibrio thiocyanoxidans ARh2
-
-
-
Thioalkalivibrio thiocyanoxidans ARh4
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
DEAE-Sepharose column chromatography and Superdex 200 gel filtration
Thioalkalivibrio paradoxus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed
758251
Thioalkalivibrio paradoxus
?
-
-
-
-
additional information
low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed
758251
Thioalkalivibrio thiocyanoxidans
?
-
-
-
-
additional information
low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed
758251
Thioalkalivibrio paradoxus ARh1
?
-
-
-
-
additional information
low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed
758251
Thioalkalivibrio thiocyanoxidans ARh2
?
-
-
-
-
additional information
low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed
758251
Thioalkalivibrio thiocyanoxidans ARh4
?
-
-
-
-
thiocyanate + 2 ferricytochrome c 550 + H2O
-
758251
Thioalkalivibrio paradoxus
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
758251
Thioalkalivibrio thiocyanoxidans
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
758251
Thioalkalivibrio paradoxus ARh1
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh2
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh4
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
-
?
thiocyanate + 2 potassium ferricyanide + H2O
-
758251
Thioalkalivibrio paradoxus
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
-
-
?
thiocyanate + 2 potassium ferricyanide + H2O
-
758251
Thioalkalivibrio thiocyanoxidans
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
-
-
?
thiocyanate + 2 potassium ferricyanide + H2O
-
758251
Thioalkalivibrio paradoxus ARh1
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
-
-
?
thiocyanate + 2 potassium ferricyanide + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh2
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
-
-
?
thiocyanate + 2 potassium ferricyanide + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh4
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio thiocyanoxidans
cyanate + sulfur + reduced Wurster's blue + 2 H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh2
cyanate + sulfur + reduced Wurster's blue + 2 H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh4
cyanate + sulfur + reduced Wurster's blue + 2 H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio paradoxus
cyanate + sulfur + reduced Wurster's blue + H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio thiocyanoxidans
cyanate + sulfur + reduced Wurster's blue + H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio paradoxus ARh1
cyanate + sulfur + reduced Wurster's blue + H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh2
cyanate + sulfur + reduced Wurster's blue + H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh4
cyanate + sulfur + reduced Wurster's blue + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 51891, calculated from amino acid sequence
Thioalkalivibrio paradoxus
homodimer
2 * 51891, calculated from amino acid sequence
Thioalkalivibrio thiocyanoxidans
homodimer
2 * 52000-53000, SDS-PAGE
Thioalkalivibrio paradoxus
homodimer
2 * 52000-53000, SDS-PAGE
Thioalkalivibrio thiocyanoxidans
Synonyms
Synonyms
Commentary
Organism
TcDH
-
Thioalkalivibrio paradoxus
TcDH
-
Thioalkalivibrio thiocyanoxidans
thiocyanate dehydrogenase
-
Thioalkalivibrio paradoxus
thiocyanate dehydrogenase
-
Thioalkalivibrio thiocyanoxidans
thiocyanate: cytochrome c oxidoreductase
-
Thioalkalivibrio paradoxus
thiocyanate: cytochrome c oxidoreductase
-
Thioalkalivibrio thiocyanoxidans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Thioalkalivibrio paradoxus
9.5
-
-
Thioalkalivibrio thiocyanoxidans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
11
more than 50% activity between pH 8.0 and 11.0 with potassium ferricyanide as electron acceptor, more than 50% activity between pH 8.5 and 10.5 with potassium ferricyanide as electron acceptor
Thioalkalivibrio paradoxus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0014
-
cyanide
at pH 9.5 and 30°C
Thioalkalivibrio paradoxus
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
60
-
at pH 9.5 and 30°C
Thioalkalivibrio paradoxus
azide
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 Star (DE3) cells
Thioalkalivibrio paradoxus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.17 M ammonium acetate, 0.085 M sodium citrate, pH 5.6, 25.5% (w/v) PEG 4000, 15% (v/v) glycerol or 0.17 M ammonium sulfate, 0.085 M sodium cacodylate, pH 6.5, 25.5% (w/v) PEG 8000, 15% (v/v) glycerol
Thioalkalivibrio paradoxus
Engineering (protein specific)
Protein Variants
Commentary
Organism
E288A
inactive
Thioalkalivibrio paradoxus
H136A
inactive
Thioalkalivibrio paradoxus
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
60
-
at pH 9.5 and 30°C
Thioalkalivibrio paradoxus
azide
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
azide
-
Thioalkalivibrio paradoxus
azide
-
Thioalkalivibrio thiocyanoxidans
Cyanate
mixed-type inhibition
Thioalkalivibrio paradoxus
Cyanate
mixed-type inhibition
Thioalkalivibrio thiocyanoxidans
cyanide
competitive inhibition
Thioalkalivibrio paradoxus
cyanide
competitive inhibition
Thioalkalivibrio thiocyanoxidans
additional information
nitrate, nitrite, bicarbonate, sulfate, acetate, and iodide show no inhibitory effect at 1 mM
Thioalkalivibrio paradoxus
additional information
nitrate, nitrite, bicarbonate, sulfate, acetate, and iodide show no inhibitory effect at 1 mM
Thioalkalivibrio thiocyanoxidans
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0014
-
cyanide
at pH 9.5 and 30°C
Thioalkalivibrio paradoxus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.8
-
Wurster's blue
at pH 9.5 and 30°C
Thioalkalivibrio paradoxus
1
-
ferricytochrome c 550
at pH 9.5 and 30°C
Thioalkalivibrio paradoxus
1.2
-
potassium ferricyanide
at pH 9.5 and 30°C
Thioalkalivibrio paradoxus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
contains three copper ions
Thioalkalivibrio paradoxus
Cu2+
contains three copper ions
Thioalkalivibrio thiocyanoxidans
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-Sepharose column chromatography and Superdex 200 gel filtration
Thioalkalivibrio paradoxus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed
758251
Thioalkalivibrio paradoxus
?
-
-
-
-
additional information
low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed
758251
Thioalkalivibrio thiocyanoxidans
?
-
-
-
-
additional information
low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed
758251
Thioalkalivibrio paradoxus ARh1
?
-
-
-
-
additional information
low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed
758251
Thioalkalivibrio thiocyanoxidans ARh2
?
-
-
-
-
additional information
low-potential electron acceptors, such as 2,3,5-triphenyltetrazolium chloride and methylene blue, are not reduced. Direct oxidation of thiocyanate by molecular oxygen is not observed
758251
Thioalkalivibrio thiocyanoxidans ARh4
?
-
-
-
-
thiocyanate + 2 ferricytochrome c 550 + H2O
-
758251
Thioalkalivibrio paradoxus
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
758251
Thioalkalivibrio thiocyanoxidans
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
758251
Thioalkalivibrio paradoxus ARh1
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh2
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh4
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
-
?
thiocyanate + 2 potassium ferricyanide + H2O
-
758251
Thioalkalivibrio paradoxus
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
-
-
?
thiocyanate + 2 potassium ferricyanide + H2O
-
758251
Thioalkalivibrio thiocyanoxidans
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
-
-
?
thiocyanate + 2 potassium ferricyanide + H2O
-
758251
Thioalkalivibrio paradoxus ARh1
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
-
-
?
thiocyanate + 2 potassium ferricyanide + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh2
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
-
-
?
thiocyanate + 2 potassium ferricyanide + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh4
cyanate + sulfur + 2 potassium ferrocyanide + 2 H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio thiocyanoxidans
cyanate + sulfur + reduced Wurster's blue + 2 H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh2
cyanate + sulfur + reduced Wurster's blue + 2 H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh4
cyanate + sulfur + reduced Wurster's blue + 2 H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio paradoxus
cyanate + sulfur + reduced Wurster's blue + H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio thiocyanoxidans
cyanate + sulfur + reduced Wurster's blue + H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio paradoxus ARh1
cyanate + sulfur + reduced Wurster's blue + H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh2
cyanate + sulfur + reduced Wurster's blue + H+
-
-
-
?
thiocyanate + Wurster's blue + H2O
-
758251
Thioalkalivibrio thiocyanoxidans ARh4
cyanate + sulfur + reduced Wurster's blue + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 51891, calculated from amino acid sequence
Thioalkalivibrio paradoxus
homodimer
2 * 51891, calculated from amino acid sequence
Thioalkalivibrio thiocyanoxidans
homodimer
2 * 52000-53000, SDS-PAGE
Thioalkalivibrio paradoxus
homodimer
2 * 52000-53000, SDS-PAGE
Thioalkalivibrio thiocyanoxidans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Thioalkalivibrio paradoxus
9.5
-
-
Thioalkalivibrio thiocyanoxidans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
11
more than 50% activity between pH 8.0 and 11.0 with potassium ferricyanide as electron acceptor, more than 50% activity between pH 8.5 and 10.5 with potassium ferricyanide as electron acceptor
Thioalkalivibrio paradoxus
Other publictions for EC 1.8.2.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
758251
Tikhonova
Trinuclear copper biocatalyti ...
Thioalkalivibrio paradoxus, Thioalkalivibrio thiocyanoxidans, Thioalkalivibrio paradoxus ARh1, Thioalkalivibrio thiocyanoxidans ARh2, Thioalkalivibrio thiocyanoxidans ARh4
Proc. Natl. Acad. Sci. USA
117
5280-5290
2020
-
-
1
1
2
-
8
3
-
2
-
-
-
13
-
-
1
-
-
-
-
-
23
4
6
-
-
-
-
2
1
-
-
1
-
1
-
-
1
-
1
2
-
1
8
1
3
-
2
-
-
-
-
-
1
-
-
-
-
23
4
-
-
-
-
2
1
-
-
-
-
-
-
-
-
756782
Tsallagov
Comparative genomics of Thioh ...
Thiohalobacter thiocyanaticus, Guyparkeria sp. SCN
Front. Microbiol.
10
898
2019
-
-
-
-
-
-
-
-
-
2
-
4
-
9
-
-
-
-
-
-
-
-
4
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
757725
Berben
Analysis of the genes involve ...
Thioalkalivibrio thiocyanoxidans, Thioalkalivibrio thiocyanoxidans ARh 2T
mSystems
2
00102-17
2017
-
-
-
-
-
-
1
-
1
1
-
2
-
2
-
-
-
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-