BRENDA - Enzyme Database
show all sequences of 1.8.2.7

Comparative genomics of Thiohalobacter thiocyanaticus HRh1T and Guyparkeria sp. SCN-R1, halophilic chemolithoautotrophic sulfur-oxidizing gammaproteobacteria capable of using thiocyanate as energy source

Tsallagov, S.I.; Sorokin, D.Y.; Tikhonova, T.V.; Popov, V.O.; Muyzer, G.; Front. Microbiol. 10, 898 (2019)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
the enzyme has a multi-copper active site
Thiohalobacter thiocyanaticus
Cu2+
the enzyme has a multi-copper active site
Guyparkeria sp. SCN
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
thiocyanate + 2 ferricyanide + H2O
Thiohalobacter thiocyanaticus
-
cyanate + sulfur + 2 ferrocyanide + 2 H+
-
-
?
thiocyanate + 2 ferricyanide + H2O
Guyparkeria sp. SCN
-
cyanate + sulfur + 2 ferrocyanide + 2 H+
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
Thiohalobacter thiocyanaticus
-
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
Guyparkeria sp. SCN
-
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Guyparkeria sp. SCN
-
formerly known as Halothiobacillus sp.
-
Thiohalobacter thiocyanaticus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
thiocyanate + 2 ferricyanide + H2O
-
756782
Thiohalobacter thiocyanaticus
cyanate + sulfur + 2 ferrocyanide + 2 H+
-
-
-
?
thiocyanate + 2 ferricyanide + H2O
-
756782
Guyparkeria sp. SCN
cyanate + sulfur + 2 ferrocyanide + 2 H+
-
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
756782
Thiohalobacter thiocyanaticus
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
756782
Guyparkeria sp. SCN
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 54000, SDS-PAGE
Thiohalobacter thiocyanaticus
Synonyms
Synonyms
Commentary
Organism
TcDH
-
Thiohalobacter thiocyanaticus
TcDH
-
Guyparkeria sp. SCN
thiocyanate dehydrogenase
-
Thiohalobacter thiocyanaticus
thiocyanate dehydrogenase
-
Guyparkeria sp. SCN
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
the enzyme has a multi-copper active site
Thiohalobacter thiocyanaticus
Cu2+
the enzyme has a multi-copper active site
Guyparkeria sp. SCN
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
thiocyanate + 2 ferricyanide + H2O
Thiohalobacter thiocyanaticus
-
cyanate + sulfur + 2 ferrocyanide + 2 H+
-
-
?
thiocyanate + 2 ferricyanide + H2O
Guyparkeria sp. SCN
-
cyanate + sulfur + 2 ferrocyanide + 2 H+
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
Thiohalobacter thiocyanaticus
-
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
Guyparkeria sp. SCN
-
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
thiocyanate + 2 ferricyanide + H2O
-
756782
Thiohalobacter thiocyanaticus
cyanate + sulfur + 2 ferrocyanide + 2 H+
-
-
-
?
thiocyanate + 2 ferricyanide + H2O
-
756782
Guyparkeria sp. SCN
cyanate + sulfur + 2 ferrocyanide + 2 H+
-
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
756782
Thiohalobacter thiocyanaticus
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
-
?
thiocyanate + 2 ferricytochrome c 550 + H2O
-
756782
Guyparkeria sp. SCN
cyanate + sulfur + 2 ferrocytochrome c 550 + 2 H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 54000, SDS-PAGE
Thiohalobacter thiocyanaticus
Other publictions for EC 1.8.2.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
758251
Tikhonova
Trinuclear copper biocatalyti ...
Thioalkalivibrio paradoxus, Thioalkalivibrio thiocyanoxidans, Thioalkalivibrio paradoxus ARh1, Thioalkalivibrio thiocyanoxidans ARh2, Thioalkalivibrio thiocyanoxidans ARh4
Proc. Natl. Acad. Sci. USA
117
5280-5290
2020
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-
1
1
2
-
8
3
-
2
-
-
-
13
-
-
1
-
-
-
-
-
23
4
6
-
-
-
-
2
1
-
-
1
-
1
-
-
1
-
1
2
-
1
8
1
3
-
2
-
-
-
-
-
1
-
-
-
-
23
4
-
-
-
-
2
1
-
-
-
-
-
-
-
-
756782
Tsallagov
Comparative genomics of Thioh ...
Thiohalobacter thiocyanaticus, Guyparkeria sp. SCN
Front. Microbiol.
10
898
2019
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-
-
-
-
-
-
-
-
2
-
4
-
9
-
-
-
-
-
-
-
-
4
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
757725
Berben
Analysis of the genes involve ...
Thioalkalivibrio thiocyanoxidans, Thioalkalivibrio thiocyanoxidans ARh 2T
mSystems
2
00102-17
2017
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-
-
-
-
-
1
-
1
1
-
2
-
2
-
-
-
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
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