Molecular analysis of dimethyl sulphide dehydrogenase from Rhodovulum sulfidophilum: Its place in the dimethyl sulphoxide reductase family of microbial molybdopterin-containing enzymes
McDevitt, C.; Hugenholtz, P.; Hanson, G.; McEwan, A.; Mol. Microbiol. 44, 1575-1587 (2002) 
Data extracted from this reference:
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Rhodovulum sulfidophilum
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
the enzyme contains 17 mol iron per mol enzyme; the enzyme contains 17 mol iron per mol enzyme; the enzyme contains 17 mol iron per mol enzyme
Rhodovulum sulfidophilum
Molybdenum
the enzyme contains 0.5 mol molybdenum per mol enzyme; the enzyme contains 0.5 mol molybdenum per mol enzyme; the enzyme contains 0.5 mol molybdenum per mol enzyme
Rhodovulum sulfidophilum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
1 * 94000 + 1 * 38000 + 1 * 32000; 1 * 94000 + 1 * 38000 + 1 * 32000; subunit DdhC
Rhodovulum sulfidophilum
38000
-
1 * 94000 + 1 * 38000 + 1 * 32000; 1 * 94000 + 1 * 38000 + 1 * 32000; subunit DdhB
Rhodovulum sulfidophilum
94000
-
1 * 94000 + 1 * 38000 + 1 * 32000; 1 * 94000 + 1 * 38000 + 1 * 32000; subunit DdhA
Rhodovulum sulfidophilum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dimethyl sulfide + ferricytochrome c2 + H2O
Rhodovulum sulfidophilum
-
dimethyl sulfoxide + ferrocytochrome c2
-
-
?
dimethyl sulfide + ferricytochrome c2 + H2O
Rhodovulum sulfidophilum SH1
-
dimethyl sulfoxide + ferrocytochrome c2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Rhodovulum sulfidophilum
Q8GPG1
subunit gamma (DdhC)
-
Rhodovulum sulfidophilum
Q8GPG3
subunit beta (DdhB)
-
Rhodovulum sulfidophilum
Q8GPG4
subunit alpha (DdhA)
-
Rhodovulum sulfidophilum SH1
Q8GPG1
subunit gamma (DdhC)
-
Rhodovulum sulfidophilum SH1
Q8GPG3
subunit beta (DdhB)
-
Rhodovulum sulfidophilum SH1
Q8GPG4
subunit alpha (DdhA)
-
Purification (Commentary)
Purification (Commentary)
Organism
Poros 20HQ Strong anion exchange column chromatography and gel filtration; Poros 20HQ Strong anion exchange column chromatography and gel filtration; Poros 20HQ Strong anion exchange column chromatography and gel filtration
Rhodovulum sulfidophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
dimethyl sulfide + ferricytochrome c2 + H2O
-
716248
Rhodovulum sulfidophilum
dimethyl sulfoxide + ferrocytochrome c2
-
-
-
?
dimethyl sulfide + ferricytochrome c2 + H2O
-
716248
Rhodovulum sulfidophilum SH1
dimethyl sulfoxide + ferrocytochrome c2
-
-
-
?
dimethyl sulfide + oxidized 2,6-dichlorophenolindophenol + H2O
-
716248
Rhodovulum sulfidophilum
dimethyl sulfoxide + reduced 2,6-dichlorophenolindophenol
-
-
-
?
dimethyl sulfide + oxidized 2,6-dichlorophenolindophenol + H2O
-
716248
Rhodovulum sulfidophilum SH1
dimethyl sulfoxide + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterotrimer
1 * 94000 + 1 * 38000 + 1 * 32000; 1 * 94000 + 1 * 38000 + 1 * 32000; 1 * 94000 + 1 * 38000 + 1 * 32000
Rhodovulum sulfidophilum
Synonyms
Synonyms
Commentary
Organism
DdhA
subunit
Rhodovulum sulfidophilum
DdhB
subunit
Rhodovulum sulfidophilum
DdhC
subunit
Rhodovulum sulfidophilum
dimethyl sulphide dehydrogenase
-
Rhodovulum sulfidophilum
DMS dehydrogenase
-
Rhodovulum sulfidophilum
DMS DH
-
Rhodovulum sulfidophilum
Cofactor
Cofactor
Commentary
Organism
Structure
molybdopterin
bis(molybdopterin guanine dinucleotide)Mo; bis(molybdopterin guanine dinucleotide)Mo; bis(molybdopterin guanine dinucleotide)Mo
Rhodovulum sulfidophilum
[3Fe-4S]-center
subunit DdhB binds one 3Fe-4S cluster
Rhodovulum sulfidophilum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
molybdopterin
bis(molybdopterin guanine dinucleotide)Mo
Rhodovulum sulfidophilum
[3Fe-4S]-center
subunit DdhB binds one 3Fe-4S cluster
Rhodovulum sulfidophilum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Rhodovulum sulfidophilum
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
the enzyme contains 17 mol iron per mol enzyme
Rhodovulum sulfidophilum
Molybdenum
the enzyme contains 0.5 mol molybdenum per mol enzyme
Rhodovulum sulfidophilum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
subunit DdhC
Rhodovulum sulfidophilum
32000
-
1 * 94000 + 1 * 38000 + 1 * 32000
Rhodovulum sulfidophilum
38000
-
1 * 94000 + 1 * 38000 + 1 * 32000
Rhodovulum sulfidophilum
38000
-
subunit DdhB
Rhodovulum sulfidophilum
94000
-
1 * 94000 + 1 * 38000 + 1 * 32000
Rhodovulum sulfidophilum
94000
-
subunit DdhA
Rhodovulum sulfidophilum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dimethyl sulfide + ferricytochrome c2 + H2O
Rhodovulum sulfidophilum
-
dimethyl sulfoxide + ferrocytochrome c2
-
-
?
dimethyl sulfide + ferricytochrome c2 + H2O
Rhodovulum sulfidophilum SH1
-
dimethyl sulfoxide + ferrocytochrome c2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Poros 20HQ Strong anion exchange column chromatography and gel filtration
Rhodovulum sulfidophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
dimethyl sulfide + ferricytochrome c2 + H2O
-
716248
Rhodovulum sulfidophilum
dimethyl sulfoxide + ferrocytochrome c2
-
-
-
?
dimethyl sulfide + ferricytochrome c2 + H2O
-
716248
Rhodovulum sulfidophilum SH1
dimethyl sulfoxide + ferrocytochrome c2
-
-
-
?
dimethyl sulfide + oxidized 2,6-dichlorophenolindophenol + H2O
-
716248
Rhodovulum sulfidophilum
dimethyl sulfoxide + reduced 2,6-dichlorophenolindophenol
-
-
-
?
dimethyl sulfide + oxidized 2,6-dichlorophenolindophenol + H2O
-
716248
Rhodovulum sulfidophilum SH1
dimethyl sulfoxide + reduced 2,6-dichlorophenolindophenol
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterotrimer
1 * 94000 + 1 * 38000 + 1 * 32000
Rhodovulum sulfidophilum
General Information
General Information
Commentary
Organism
malfunction
the ddhA mutant is not able to grow photoautotrophically with dimethyl sulfide as electron donor, although photoheterotrophic growth is not impaired
Rhodovulum sulfidophilum
physiological function
dimethyl sulfide dehydrogenase is essential for photolithotrophic growth of Rhodovulum sulfidophilum on dimethyl sulfide but not for chemo-trophic growth on the same substrate; dimethyl sulfide dehydrogenase is essential for photolithotrophic growth of Rhodovulum sulfidophilum on dimethyl sulfide but not for chemo-trophic growth on the same substrate; dimethyl sulfide dehydrogenase is essential for photolithotrophic growth of Rhodovulum sulfidophilum on dimethyl sulfide but not for chemo-trophic growth on the same substrate
Rhodovulum sulfidophilum
General Information (protein specific)
General Information
Commentary
Organism
malfunction
the ddhA mutant is not able to grow photoautotrophically with dimethyl sulfide as electron donor, although photoheterotrophic growth is not impaired
Rhodovulum sulfidophilum
physiological function
dimethyl sulfide dehydrogenase is essential for photolithotrophic growth of Rhodovulum sulfidophilum on dimethyl sulfide but not for chemo-trophic growth on the same substrate
Rhodovulum sulfidophilum
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
715664
Creevey
A mechanistic and electrochemi ...
Rhodovulum sulfidophilum, Rhodovulum sulfidophilum SH1
J. Biol. Inorg. Chem.
13
1231-1238
2008
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2
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2
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5
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1
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4
1
3
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1
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3
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3
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-
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2
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2
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1
-
-
-
-
4
1
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-
-
-
1
-
-
-
-
-
-
-
-
-
716248
McDevitt
Molecular analysis of dimethyl ...
Rhodovulum sulfidophilum, Rhodovulum sulfidophilum SH1
Mol. Microbiol.
44
1575-1587
2002
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-
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1
2
3
6
-
9
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1
-
-
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12
1
12
-
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2
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4
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-
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3
6
9
6
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3
-
-
-
-
12
3
-
-
-
-
-
-
-
-
-
2
4
-
-
-
714886
Hanlon
Dimethylsulfide: acceptor oxid ...
Rhodovulum sulfidophilum
Eur. J. Biochem.
239
391-396
1996
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1
2
4
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1
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1
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2
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5
1
2
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3
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3
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1
2
4
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1
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2
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5
1
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