BRENDA - Enzyme Database
show all sequences of 1.8.2.4

A mechanistic and electrochemical study of the interaction between dimethyl sulfide dehydrogenase and its electron transfer partner cytochrome c2

Creevey, N.; McEwan, A.; Bernhardt, P.; J. Biol. Inorg. Chem. 13, 1231-1238 (2008)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
ferricytochrome c2
in 220 mM Tris-HCl pH 8.0, 22°C
Rhodovulum sulfidophilum
0.053
-
Dimethyl sulfide
in 220 mM Tris-HCl pH 8.0, 22°C
Rhodovulum sulfidophilum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dimethyl sulfide + ferricytochrome c2 + H2O
Rhodovulum sulfidophilum
-
dimethyl sulfoxide + ferrocytochrome c2
-
-
?
dimethyl sulfide + ferricytochrome c2 + H2O
Rhodovulum sulfidophilum SH1
-
dimethyl sulfoxide + ferrocytochrome c2
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Rhodovulum sulfidophilum
-
-
-
Rhodovulum sulfidophilum SH1
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
ammonium sulfate precipitation, SP-Sepharose column chromatography, gel filtration
Rhodovulum sulfidophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
dimethyl sulfide + ferricytochrome c2 + H2O
-
715664
Rhodovulum sulfidophilum
dimethyl sulfoxide + ferrocytochrome c2
-
-
-
?
dimethyl sulfide + ferricytochrome c2 + H2O
DMS:ferrocytochrome oxidoreductase operates via a two-site ping-pong mechanism
715664
Rhodovulum sulfidophilum
dimethyl sulfoxide + ferrocytochrome c2
-
-
-
?
dimethyl sulfide + ferricytochrome c2 + H2O
-
715664
Rhodovulum sulfidophilum SH1
dimethyl sulfoxide + ferrocytochrome c2
-
-
-
?
dimethyl sulfide + ferricytochrome c2 + H2O
DMS:ferrocytochrome oxidoreductase operates via a two-site ping-pong mechanism
715664
Rhodovulum sulfidophilum SH1
dimethyl sulfoxide + ferrocytochrome c2
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterotrimer
-
Rhodovulum sulfidophilum
Synonyms
Synonyms
Commentary
Organism
dimethyl sulfide dehydrogenase
-
Rhodovulum sulfidophilum
DMS dehydrogenase
-
Rhodovulum sulfidophilum
DMS:ferricytochrome c2 oxidoreductase
-
Rhodovulum sulfidophilum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Rhodovulum sulfidophilum
Cofactor
Cofactor
Commentary
Organism
Structure
heme
the enzyme contains a heme b cofactor
Rhodovulum sulfidophilum
iron-sulfur centre
the enzyme contains five iron-sulfur clusters
Rhodovulum sulfidophilum
molybdopterin
-
Rhodovulum sulfidophilum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
the enzyme contains a heme b cofactor
Rhodovulum sulfidophilum
iron-sulfur centre
the enzyme contains five iron-sulfur clusters
Rhodovulum sulfidophilum
molybdopterin
-
Rhodovulum sulfidophilum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
ferricytochrome c2
in 220 mM Tris-HCl pH 8.0, 22°C
Rhodovulum sulfidophilum
0.053
-
Dimethyl sulfide
in 220 mM Tris-HCl pH 8.0, 22°C
Rhodovulum sulfidophilum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dimethyl sulfide + ferricytochrome c2 + H2O
Rhodovulum sulfidophilum
-
dimethyl sulfoxide + ferrocytochrome c2
-
-
?
dimethyl sulfide + ferricytochrome c2 + H2O
Rhodovulum sulfidophilum SH1
-
dimethyl sulfoxide + ferrocytochrome c2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, SP-Sepharose column chromatography, gel filtration
Rhodovulum sulfidophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
dimethyl sulfide + ferricytochrome c2 + H2O
-
715664
Rhodovulum sulfidophilum
dimethyl sulfoxide + ferrocytochrome c2
-
-
-
?
dimethyl sulfide + ferricytochrome c2 + H2O
DMS:ferrocytochrome oxidoreductase operates via a two-site ping-pong mechanism
715664
Rhodovulum sulfidophilum
dimethyl sulfoxide + ferrocytochrome c2
-
-
-
?
dimethyl sulfide + ferricytochrome c2 + H2O
-
715664
Rhodovulum sulfidophilum SH1
dimethyl sulfoxide + ferrocytochrome c2
-
-
-
?
dimethyl sulfide + ferricytochrome c2 + H2O
DMS:ferrocytochrome oxidoreductase operates via a two-site ping-pong mechanism
715664
Rhodovulum sulfidophilum SH1
dimethyl sulfoxide + ferrocytochrome c2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterotrimer
-
Rhodovulum sulfidophilum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Rhodovulum sulfidophilum
Other publictions for EC 1.8.2.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
715664
Creevey
A mechanistic and electrochemi ...
Rhodovulum sulfidophilum, Rhodovulum sulfidophilum SH1
J. Biol. Inorg. Chem.
13
1231-1238
2008
-
-
-
-
-
-
-
2
-
-
-
2
-
9
-
-
1
-
-
-
-
-
4
1
3
-
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
716248
McDevitt
Molecular analysis of dimethyl ...
Rhodovulum sulfidophilum, Rhodovulum sulfidophilum SH1
Mol. Microbiol.
44
1575-1587
2002
-
-
-
-
-
-
-
-
3
6
9
6
-
13
-
-
3
-
-
-
-
-
12
3
12
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
3
6
9
6
-
-
-
3
-
-
-
-
12
3
-
-
-
-
-
-
-
-
-
4
4
-
-
-
714886
Hanlon
Dimethylsulfide: acceptor oxid ...
Rhodovulum sulfidophilum
Eur. J. Biochem.
239
391-396
1996
-
-
-
-
-
-
-
-
1
2
4
-
-
1
-
-
1
-
-
-
2
-
5
1
2
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
2
4
-
-
-
-
1
-
-
2
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-