BRENDA - Enzyme Database show
show all sequences of 1.8.2.4

Dimethylsulfide: acceptor oxidoreductase from Rhodobacter sulfidophilus - The purified enzyme contains b-type haem and a pterin molybdenum cofactor

Hanlon, S.; Toh, T.; Solomon, P.; Holt, R.; McEwan, A.; Eur. J. Biochem. 239, 391-396 (1996)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Rhodovulum sulfidophilum
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe
the enzyme contains 3.5 mol Fe per mol enzyme
Rhodovulum sulfidophilum
Molybdenum
the enzyme contains 0.5 mol molybdenum per mol enzyme
Rhodovulum sulfidophilum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE
Rhodovulum sulfidophilum
38000
-
1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE
Rhodovulum sulfidophilum
94000
-
1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE
Rhodovulum sulfidophilum
152000
-
gel filtration
Rhodovulum sulfidophilum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodovulum sulfidophilum
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, and Sephacryl S-200 gel filtration
Rhodovulum sulfidophilum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
18.2
-
crude extract, using reduced 2,6-dichloroindophenol as cosubstrate, in 50 mM Tris/HCI pH 8.0, temperature not specified in the publication
Rhodovulum sulfidophilum
20.2
-
after purification, using reduced 2,6-dichloroindophenol as cosubstrate, in 50 mM Tris/HCI pH 8.0, temperature not specified in the publication
Rhodovulum sulfidophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dimethyl sulfoxide + reduced 2,6-dichloroindophenol
-
714886
Rhodovulum sulfidophilum
dimethyl sulfide + oxidized 2,6-dichloroindophenol + H2O
-
-
-
?
dimethyl sulfoxide + reduced methyl viologen + H2O
2% activity compared to trimethylamine N-oxide
714886
Rhodovulum sulfidophilum
dimethyl sulfide + oxidized methyl viologen
-
-
-
?
lauryldimethylamine N-oxide + reduced methyl viologen + H2O
69% activity compared to trimethylamine N-oxide
714886
Rhodovulum sulfidophilum
?
-
-
-
?
additional information
no activity with chlorate and bromate
714886
Rhodovulum sulfidophilum
?
-
-
-
-
trimethylamine N-oxide + reduced methyl viologen + H2O
100% activity
714886
Rhodovulum sulfidophilum
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterotrimer
1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE
Rhodovulum sulfidophilum
Cofactor
Cofactor
Commentary
Organism
Structure
heme
the cytochrome contains a b-type heme and a content of 0.65 mol protoheme per mol enzyme is determined
Rhodovulum sulfidophilum
iron-sulfur centre
-
Rhodovulum sulfidophilum
molybdopterin
-
Rhodovulum sulfidophilum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
the cytochrome contains a b-type heme and a content of 0.65 mol protoheme per mol enzyme is determined
Rhodovulum sulfidophilum
iron-sulfur centre
-
Rhodovulum sulfidophilum
molybdopterin
-
Rhodovulum sulfidophilum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Rhodovulum sulfidophilum
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe
the enzyme contains 3.5 mol Fe per mol enzyme
Rhodovulum sulfidophilum
Molybdenum
the enzyme contains 0.5 mol molybdenum per mol enzyme
Rhodovulum sulfidophilum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE
Rhodovulum sulfidophilum
38000
-
1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE
Rhodovulum sulfidophilum
94000
-
1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE
Rhodovulum sulfidophilum
152000
-
gel filtration
Rhodovulum sulfidophilum
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, and Sephacryl S-200 gel filtration
Rhodovulum sulfidophilum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
18.2
-
crude extract, using reduced 2,6-dichloroindophenol as cosubstrate, in 50 mM Tris/HCI pH 8.0, temperature not specified in the publication
Rhodovulum sulfidophilum
20.2
-
after purification, using reduced 2,6-dichloroindophenol as cosubstrate, in 50 mM Tris/HCI pH 8.0, temperature not specified in the publication
Rhodovulum sulfidophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dimethyl sulfoxide + reduced 2,6-dichloroindophenol
-
714886
Rhodovulum sulfidophilum
dimethyl sulfide + oxidized 2,6-dichloroindophenol + H2O
-
-
-
?
dimethyl sulfoxide + reduced methyl viologen + H2O
2% activity compared to trimethylamine N-oxide
714886
Rhodovulum sulfidophilum
dimethyl sulfide + oxidized methyl viologen
-
-
-
?
lauryldimethylamine N-oxide + reduced methyl viologen + H2O
69% activity compared to trimethylamine N-oxide
714886
Rhodovulum sulfidophilum
?
-
-
-
?
additional information
no activity with chlorate and bromate
714886
Rhodovulum sulfidophilum
?
-
-
-
-
trimethylamine N-oxide + reduced methyl viologen + H2O
100% activity
714886
Rhodovulum sulfidophilum
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterotrimer
1 * 94000 + 1 * 38000 + 1 * 32000, SDS-PAGE
Rhodovulum sulfidophilum
Other publictions for EC 1.8.2.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
715664
Creevey
A mechanistic and electrochemi ...
Rhodovulum sulfidophilum, Rhodovulum sulfidophilum SH1
J. Biol. Inorg. Chem.
13
1231-1238
2008
-
-
-
-
-
-
-
2
-
-
-
2
-
3
-
-
1
-
-
-
-
-
4
1
-
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
716248
McDevitt
Molecular analysis of dimethyl ...
Rhodovulum sulfidophilum, Rhodovulum sulfidophilum SH1
Mol. Microbiol.
44
1575-1587
2002
-
-
-
-
-
-
-
-
1
2
3
6
-
7
-
-
1
-
-
-
-
-
12
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
-
-
-
-
-
3
6
9
6
-
-
-
3
-
-
-
-
12
3
-
-
-
-
-
-
-
-
-
2
4
-
-
-
714886
Hanlon
Dimethylsulfide: acceptor oxid ...
Rhodovulum sulfidophilum
Eur. J. Biochem.
239
391-396
1996
-
-
-
-
-
-
-
-
1
2
4
-
-
1
-
-
1
-
-
-
2
-
5
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
2
4
-
-
-
-
1
-
-
2
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
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-