BRENDA - Enzyme Database
show all sequences of 1.8.2.3

A novel membrane-bound flavocytochrome c sulfide dehydrogenase from the colourless sulfur bacterium Thiobacillus sp. W5

Visser, J.; De Jong, G.; Robertson, L.; Kuenen, J.; Arch. Microbiol. 167, 295-301 (1997)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
cyanide
-
Thiobacillus sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0017
-
Sulfide
pH and temperature not specified in the publication
Thiobacillus sp.
0.0038
-
oxidized cytochrome c
pH and temperature not specified in the publication
Thiobacillus sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Thiobacillus sp.
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
11000
-
1 * 40000 + 1 * 11000, SDS-PAGE
Thiobacillus sp.
40000
-
1 * 40000 + 1 * 11000, SDS-PAGE
Thiobacillus sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thiobacillus sp.
-
-
-
Thiobacillus sp. W5
-
-
-
Purification (Commentary)
Commentary
Organism
-
Thiobacillus sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sulfide + oxidized cytochrome c
-
713981
Thiobacillus sp.
sulfur + reduced cytochrome c + H+
-
-
-
?
sulfide + oxidized cytochrome c
-
713981
Thiobacillus sp. W5
sulfur + reduced cytochrome c + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterodimer
1 * 40000 + 1 * 11000, SDS-PAGE
Thiobacillus sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
-
Thiobacillus sp.
Cofactor
Cofactor
Commentary
Organism
Structure
flavin
the enzyme contains one molecule flavin
Thiobacillus sp.
heme
the smaller subunit contains one molecule covalently bound heme c
Thiobacillus sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
flavin
the enzyme contains one molecule flavin
Thiobacillus sp.
heme
the smaller subunit contains one molecule covalently bound heme c
Thiobacillus sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cyanide
-
Thiobacillus sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0017
-
Sulfide
pH and temperature not specified in the publication
Thiobacillus sp.
0.0038
-
oxidized cytochrome c
pH and temperature not specified in the publication
Thiobacillus sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Thiobacillus sp.
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
11000
-
1 * 40000 + 1 * 11000, SDS-PAGE
Thiobacillus sp.
40000
-
1 * 40000 + 1 * 11000, SDS-PAGE
Thiobacillus sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thiobacillus sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sulfide + oxidized cytochrome c
-
713981
Thiobacillus sp.
sulfur + reduced cytochrome c + H+
-
-
-
?
sulfide + oxidized cytochrome c
-
713981
Thiobacillus sp. W5
sulfur + reduced cytochrome c + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterodimer
1 * 40000 + 1 * 11000, SDS-PAGE
Thiobacillus sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.6
-
-
Thiobacillus sp.
Other publictions for EC 1.8.2.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
714154
Quentmeier
Sulfide dehydrogenase activity ...
Paracoccus pantotrophus
Biochemistry
43
14696-14703
2004
-
-
-
-
-
-
4
2
-
-
3
-
-
1
-
-
1
-
-
-
-
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2
1
-
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-
1
1
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-
1
2
-
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-
-
1
-
-
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4
2
2
-
-
3
-
-
-
-
1
-
-
-
-
2
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
715342
Kostanjevecki
A membrane-bound flavocytochro ...
Ectothiorhodospira shaposhnikovii
J. Bacteriol.
182
3097-3103
2000
-
-
-
-
-
-
-
-
1
-
2
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-
6
-
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1
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-
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1
1
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1
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1
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-
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-
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1
-
2
-
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-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
2
-
-
713981
Visser
A novel membrane-bound flavocy ...
Thiobacillus sp., Thiobacillus sp. W5
Arch. Microbiol.
167
295-301
1997
-
-
-
-
-
-
1
2
1
-
2
-
-
7
-
-
1
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-
-
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2
1
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1
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2
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2
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-
1
-
2
1
-
2
-
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-
1
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-
2
1
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-
-
-
1
-
-
-
-
-
-
-
-
-
716866
Van Driessche
Covalent structure of the flav ...
Allochromatium vinosum
Protein Sci.
5
1753-1764
1996
-
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
1
-
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-
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-
1
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-
1
-
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-
1
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-
2
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-
1
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
716105
Yamanaka
-
Sulfide-cytochrome c reductase ...
Allochromatium vinosum, Chlorobaculum thiosulfatiphilum
Methods Enzymol.
243
463-472
1994
-
-
-
-
-
-
2
-
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6
-
-
2
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-
2
-
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-
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4
2
-
-
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4
-
2
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-
4
-
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-
2
-
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-
-
6
-
-
-
-
2
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-
-
4
2
-
-
-
-
-
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-
2
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-
716916
Chen
The structure of flavocytochro ...
Allochromatium vinosum
Science
266
430-432
1994
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-
-
1
-
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3
-
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4
-
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-
1
1
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2
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2
1
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3
-
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1
1
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-
714363
Gray
-
The role of a cytochrome c-552 ...
Allochromatium vinosum
Biochim. Biophys. Acta
680
290-296
1982
-
-
-
-
-
-
-
-
-
-
4
-
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1
-
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-
1
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2
1
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-
-
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-
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4
-
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-
1
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-
2
1
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-
-
-
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-
-
-
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-
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715409
Fukumori
Flavocytochrome c of Chromatiu ...
Allochromatium vinosum
J. Biochem.
85
1405-1414
1979
-
-
-
-
-
-
2
1
-
-
3
-
-
5
-
-
-
-
-
-
-
-
5
1
-
-
1
-
1
-
-
-
-
-
-
-
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-
-
-
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-
-
2
-
1
-
-
3
-
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-
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-
-
-
-
-
5
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
714361
Kusai
The oxidation mechanisms of th ...
Chlorobium vibrioforme f. thiosulfatophilum
Biochim. Biophys. Acta
325
304-314
1973
1
-
-
-
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-
4
-
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1
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1
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1
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1
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2
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1
1
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2
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1
4
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1
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1
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1
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