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Literature summary for 1.8.2.2 extracted from

  • Kikumoto, M.; Nogami, S.; Kanao, T.; Takada, J.; Kamimura, K.
    Tetrathionate-forming thiosulfate dehydrogenase from the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans (2013), Appl. Environ. Microbiol., 79, 113-120.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
sulfite 2 mM, 1.6fold stimulation. Inhibitory at higher concentrations, almost complete inhibition at 10 mM Acidithiobacillus ferrooxidans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Acidithiobacillus ferrooxidans

Inhibitors

Inhibitors Comment Organism Structure
sulfate 200 mM, about 60% reduction in activity Acidithiobacillus ferrooxidans
sulfite 2 mM, 1.6fold stimulation. Inhibitory at higher concentrations, almost complete inhibition at 10 mM Acidithiobacillus ferrooxidans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8
-
thiosulfate recombinant protein, pH 2.5, 50°C Acidithiobacillus ferrooxidans
15
-
thiosulfate native protein, pH 2.5, 50°C Acidithiobacillus ferrooxidans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
x * 25000, SDS-PAGE, x * 25796, calculated, mature protein Acidithiobacillus ferrooxidans
25796
-
x * 25000, SDS-PAGE, x * 25796, calculated, mature protein Acidithiobacillus ferrooxidans

Organism

Organism UniProt Comment Textmining
Acidithiobacillus ferrooxidans B7J3E3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a signal peptide of 37 amino acids Acidithiobacillus ferrooxidans

Purification (Commentary)

Purification (Comment) Organism
partial Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.16
-
pH 2.5, 40°C, recombinant protein Acidithiobacillus ferrooxidans
6.5
-
native protein, pH 2.5, 50°C Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 thiosulfate + 2 ferricyanide no catalytic activity below 4 mM thiosulfate. Enzyme catalyzes the formation of 1 mol of tetrathionate from 2 mol of thiosulfate Acidithiobacillus ferrooxidans tetrathionate + 2 ferrocyanide
-
?
additional information no substrates: sulfite, ubiquinone and horse heart cytochrome c Acidithiobacillus ferrooxidans ?
-
?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE, x * 25796, calculated, mature protein Acidithiobacillus ferrooxidans

Synonyms

Synonyms Comment Organism
AFE_0042 locus name Acidithiobacillus ferrooxidans
Tat pathway signal sequence domain protein
-
Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
recombinant protein Acidithiobacillus ferrooxidans
70
-
native protein Acidithiobacillus ferrooxidans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
-
Acidithiobacillus ferrooxidans

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: heme Acidithiobacillus ferrooxidans

pI Value

Organism Comment pI Value Maximum pI Value
Acidithiobacillus ferrooxidans calculated, mature protein
-
6.2

General Information

General Information Comment Organism
metabolism enzyme activity is detected in strains grown on tetrathionate or sulfur, but no activity is detected in ferrous iron-grown cells Acidithiobacillus ferrooxidans