Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-bipyridyl | 50% inhibition at 1 mM, a 60 min incubation period is necessary to potentiate the inhibition | Thiobacillus thioparus | |
EDTA | 44% inhibition at 10 mM, a 15 min incubation period is necessary to potentiate the inhibition | Thiobacillus thioparus | |
HgCl2 | 100% inhibition at 0.5 mM | Thiobacillus thioparus | |
N-ethylmaleimide | 100% inhibition at 1 mM, a 15 min incubation period is necessary to potentiate the inhibition | Thiobacillus thioparus | |
p-chloromercuribenzoate | 72.8% inhibition at 1 mM | Thiobacillus thioparus | |
SO32- | 50% inhibition at 0.005 mM , time-dependent inhibition, irreversible | Thiobacillus thioparus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Thiobacillus thioparus | |
0.005 | - |
thiosulfate | cytochrome c as electron acceptor | Thiobacillus thioparus | |
0.1 | - |
thiosulfate | ferricyanide as electron acceptor | Thiobacillus thioparus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | enzyme contains 2 mol of non-heme iron | Thiobacillus thioparus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
sucrose density gradient method | Thiobacillus thioparus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thiobacillus thioparus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using sonication, column chromatography on DEAE-cellulose, pH-adjustment to 4.5 and calcium phosphate gel treatment | Thiobacillus thioparus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
146.1 | - |
- |
Thiobacillus thioparus |
Storage Stability | Organism |
---|---|
-20°C, the ability of the enzyme to couple with cytochrome c decreases during storage | Thiobacillus thioparus |
4°C, 10% loss of activity after 6 h and 50% loss of activity after 5 days | Thiobacillus thioparus |
frozen, stable, indefinitely as measured by ferricyanide reduction | Thiobacillus thioparus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NAD+, methylene blue, 2,6-dichlorophenol indophenol | Thiobacillus thioparus | ? | - |
? | |
additional information | no activity with: NADP+, O2 | Thiobacillus thioparus | ? | - |
? | |
thiosulfate + oxidized acceptor | cytochrome c of yeast is a poor electron acceptor | Thiobacillus thioparus | tetrathionate + reduced acceptor | - |
? | |
thiosulfate + oxidized acceptor | horse cytochrome c is a poor electron acceptor | Thiobacillus thioparus | tetrathionate + reduced acceptor | - |
? | |
thiosulfate + oxidized acceptor | cytochrome c acts as electron acceptor | Thiobacillus thioparus | tetrathionate + reduced acceptor | - |
? | |
thiosulfate + oxidized acceptor | ferricyanide is a much better electron acceptor than cytochrome c | Thiobacillus thioparus | tetrathionate + reduced acceptor | - |
? | |
thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Thiobacillus thioparus | tetrathionate + reduced acceptor | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
6 h, 10% loss of activity, 50% loss of activity after 5 days | Thiobacillus thioparus |
55 | - |
5 min, stable | Thiobacillus thioparus |
60 | - |
5 min, 50% loss of activity | Thiobacillus thioparus |
100 | - |
1 min, complete loss of activity | Thiobacillus thioparus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
below, using ferricyanide as electron acceptor | Thiobacillus thioparus |
6.5 | - |
broad, with cytochrome c as electron acceptor | Thiobacillus thioparus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.8 | little change in activity between | Thiobacillus thioparus |