KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | temperature dependency of Km | Pseudomonas sp. | |
1.57 | - |
thiosulfate | pH 6.1-6.3, 30°C | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
16B, marine heterotroph | - |
Purification (Comment) | Organism |
---|---|
partial, using ion-exchange chromatography and calcium phosphate gel treatment | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.38 | - |
purified enzyme | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, 36% loss of activity after 9 days | Pseudomonas sp. |
4°C, stable, 2 days | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiosulfate + oxidized acceptor | only native cytochrome c acts as electron acceptor | Pseudomonas sp. | tetrathionate + reduced acceptor | - |
? | |
thiosulfate + oxidized acceptor | ferricyanide or native cytochrome c act as electron acceptors | Pseudomonas sp. | tetrathionate + reduced acceptor | - |
? | |
thiosulfate + oxidized acceptor | cytochrome c acts as electron acceptor | Pseudomonas sp. | tetrathionate + reduced acceptor | - |
? | |
thiosulfate + oxidized acceptor | the activity does not couple with mammalian cytochrome c as electron acceptor | Pseudomonas sp. | tetrathionate + reduced acceptor | - |
? | |
thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Pseudomonas sp. | tetrathionate + reduced acceptor | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
after 1 min the activity is completely destroyed | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
no definite pH-optimum | Pseudomonas sp. |