Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | not inhibitory at 10 mM | Thiobacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thiobacillus sp. | - |
X | - |
Thiobacillus sp. X | - |
X | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thiobacillus sp. |
Storage Stability | Organism |
---|---|
-25°C, 0.02 M phosphate, 40% loss of activity after 2 months | Thiobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiosulfate + oxidized acceptor | cytochrome c acts as electron acceptor | Thiobacillus sp. | tetrathionate + reduced acceptor | - |
ir | |
thiosulfate + oxidized acceptor | cytochrome 557 becames reduced when mixed with thiosulfate, enzyme and a trace of cytochrome 553.5, the omission of either the enzyme or cytochrome 553.5 prevents this reduction | Thiobacillus sp. | tetrathionate + reduced acceptor | - |
ir | |
thiosulfate + oxidized acceptor | mammalian cytochrome c acts as electron acceptor | Thiobacillus sp. | tetrathionate + reduced acceptor | - |
ir | |
thiosulfate + oxidized acceptor | cytochrome c553.5 acts as electron acceptor | Thiobacillus sp. | tetrathionate + reduced acceptor | - |
ir | |
thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Thiobacillus sp. | tetrathionate + reduced acceptor | - |
ir | |
thiosulfate + oxidized acceptor | cytochrome c acts as electron acceptor | Thiobacillus sp. X | tetrathionate + reduced acceptor | - |
ir | |
thiosulfate + oxidized acceptor | cytochrome 557 becames reduced when mixed with thiosulfate, enzyme and a trace of cytochrome 553.5, the omission of either the enzyme or cytochrome 553.5 prevents this reduction | Thiobacillus sp. X | tetrathionate + reduced acceptor | - |
ir | |
thiosulfate + oxidized acceptor | mammalian cytochrome c acts as electron acceptor | Thiobacillus sp. X | tetrathionate + reduced acceptor | - |
ir | |
thiosulfate + oxidized acceptor | cytochrome c553.5 acts as electron acceptor | Thiobacillus sp. X | tetrathionate + reduced acceptor | - |
ir | |
thiosulfate + oxidized acceptor | ferricyanide acts as electron acceptor | Thiobacillus sp. X | tetrathionate + reduced acceptor | - |
ir |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
3 min, pH 7, complete loss of activity | Thiobacillus sp. |
60 | - |
enzyme activity completely destroyed after 3 min at pH 7 | Thiobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
the rate of reduction of cytochrome 553.5 at pH 5 is about 10% of the rate at pH 7 | Thiobacillus sp. |
7 | - |
for the reduction of cytochrome 553.5 | Thiobacillus sp. |