Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli TP1000 cells | Gallus gallus |
Crystallization (Comment) | Organism |
---|---|
the C185S variant is crystallized by the hanging drop vapor diffusion method using 15% (v/v) 2-methyl-2,4-pentanediol, 2% PEG 4000 (w/v), and 100 mM sodium acetate (pH 5.0) and in the presence of sulfite using 2% PEG 4000 (w/v), 10% MPD (v/v), 100 mM sodium acetate (pH 5.0) and 0.5 mM sodium sulfite. The C185A variant is crystallized by the sitting drop vapor diffusion method, using 15% (w/v) PEG 4000, 150 mM (NH4)2SO4, and 100 mM MES (pH 6.0) | Gallus gallus |
Protein Variants | Comment | Organism |
---|---|---|
C185A | the active site of the mutant is essentially catalytically inactive with ferricyctochrome c or ferricyanide as electron acceptor | Gallus gallus |
C185S | the active site of the mutant is essentially catalytically inactive with ferricyctochrome c or ferricyanide as electron acceptor | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + ferricytochrome c + H2O | Gallus gallus | ferricytochrome c is the physiological electron acceptor | sulfate + ferrocytochrome c + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | P07850 | - |
- |
Purification (Comment) | Organism |
---|---|
phenyl-Sepharose column chromatography and Superdex-200 gel filtration | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + ferricyanide + H2O | - |
Gallus gallus | sulfate + ferrocyanide + H+ | - |
? | |
sulfite + ferricytochrome c + H2O | ferricytochrome c is the physiological electron acceptor | Gallus gallus | sulfate + ferrocytochrome c + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sulfate oxidase | - |
Gallus gallus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Gallus gallus | |
molybdopterin | - |
Gallus gallus |