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Literature summary for 1.8.1.B1 extracted from

  • Rendon, J.L.; Miranda-Leyva, M.; Guevara-Flores, A.; Martinez-Gonzalez, J.J.; Del Arenal, I.P.; Flores-Herrera, O.; Pardo, J.P.
    Insight into the mechanistic basis of the hysteretic-like kinetic behavior of thioredoxin-glutathione reductase (TGR) (2018), Enzyme Res., 2018, 3215462 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.1.B1

Inhibitors

Inhibitors Comment Organism Structure
glutathione disulfide substrate inhibition at moderate or high concentrations. Noncompetitive mode of inhibition in which the disulfide behaves as an affinity label-like reagent through its binding and reduction at an alternative site, leading the enzyme into an inactive state Taenia crassiceps
NADP+
-
 Taenia crassiceps

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
 NADPH pH 7.8, 25°C Taenia crassiceps
0.0144
-
 glutathione disulfide pH 7.8, 25°C Taenia crassiceps

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione disulfide + NADPH + H+ Taenia crassiceps
-
 2 glutathione + NADP+
-
 ?
thioredoxin disulfide + NADPH + H+ Taenia crassiceps
-
 thioredoxin + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Taenia crassiceps
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione disulfide + NADPH + H+
-
 Taenia crassiceps 2 glutathione + NADP+
-
 ?
glutathione disulfide + NADPH + H+ the enzyme follows a two-site ping-pong bi bi kinetic mechanism, in which both substrates and products are bound in rapid equilibrium fashion Taenia crassiceps 2 glutathione + NADP+
-
 ?
thioredoxin disulfide + NADPH + H+
-
 Taenia crassiceps thioredoxin + NADP+
-
 ?
thioredoxin disulfide + NADPH + H+ the enzyme follows a two-site ping-pong bi bi kinetic mechanism, in which both substrates and products are bound in rapid equilibrium fashion Taenia crassiceps thioredoxin + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
TGR
-
 Taenia crassiceps
thioredoxin-glutathione reductase
-
 Taenia crassiceps

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12
-
 NADPH pH 7.8, 25°C Taenia crassiceps
12
-
 glutathione disulfide pH 7.8, 25°C Taenia crassiceps

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0376
-
 NADP+ pH 7.8, 25°C, competitive inhibition regarding NADPH Taenia crassiceps
0.0553
-
 NADP+ pH 7.8, 25°C, uncompetitive inhibition regarding GSSG Taenia crassiceps
0.331
-
 glutathione disulfide pH 7.8, 25°C Taenia crassiceps

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
830
-
 glutathione disulfide pH 7.8, 25°C Taenia crassiceps
3430
-
 NADPH pH 7.8, 25°C Taenia crassiceps