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Literature summary for 1.8.1.B1 extracted from

  • Prum, S.; Plumworasawat, S.; Chaiyadet, S.; Saichua, P.; Thanan, R.; Laha, T.; Laohaviroj, M.; Sripa, B.; Suttiprapa, S.
    Characterization and in vitro functional analysis of thioredoxin glutathione reductase from the liver fluke Opisthorchis viverrini (2020), Acta Trop., 210, 105621 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the ORF of Ov-TGR is inserted into pABC2 plasmid and transformed into Escherichia coli strain C321.DETLTAA to facilitate selenocysteine incorporation Opisthorchis viverrini

Inhibitors

Inhibitors Comment Organism Structure
auranofin inhibition in a dose-dependent manner. The inhibitor expresses a lethal toxic effect on both newly excysted juveniles and adult worms of Opisthorchis viverrini in vitro Opisthorchis viverrini

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00809
-
glutathione disulfide pH 7.4, 25°C Opisthorchis viverrini
0.01374
-
thioredoxin disulfide pH 7.4, 25°C Opisthorchis viverrini

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
calculated from sequence Opisthorchis viverrini

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione disulfide + NADPH + H+ Opisthorchis viverrini
-
2 glutathione + NADP+
-
?
thioredoxin disulfide + NADPH + H+ Opisthorchis viverrini
-
thioredoxin + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Opisthorchis viverrini A0A6C0NKS2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Opisthorchis viverrini

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione disulfide + NADPH + H+
-
Opisthorchis viverrini 2 glutathione + NADP+
-
?
thioredoxin disulfide + NADPH + H+
-
Opisthorchis viverrini thioredoxin + NADP+
-
?

Subunits

Subunits Comment Organism
dimer
-
Opisthorchis viverrini

Synonyms

Synonyms Comment Organism
TGR
-
Opisthorchis viverrini
thioredoxin glutathione reductase
-
Opisthorchis viverrini

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.79
-
thioredoxin disulfide pH 7.4, 25°C Opisthorchis viverrini
61.04
-
glutathione disulfide pH 7.4, 25°C Opisthorchis viverrini

General Information

General Information Comment Organism
drug target the enzyme maybe a potential target for the development of novel agents against opisthorschiasis Opisthorchis viverrini
physiological function the enzyme is crucial for Opisthorchis viverrini survival Opisthorchis viverrini

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
640
-
thioredoxin disulfide pH 7.4, 25°C Opisthorchis viverrini
7500
-
glutathione disulfide pH 7.4, 25°C Opisthorchis viverrini