Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a six-histidine tagged protein. Recombinant His-SmTGR, which would not have a penultimate selenocysteine when expressed in Escherichia coli, has no measurable activity in the insulin reduction assay | Schistosoma mansoni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
auranofin | - |
Schistosoma mansoni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenium | selenocysteine protein | Schistosoma mansoni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schistosoma mansoni | Q962Y6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Schistosoma mansoni |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adult | thioredoxin glutathione reductase appears to be the major, if not the sole enzyme for these activities in adult worms, completely replacing thioredoxin reductase and glutathione reductase | Schistosoma mansoni | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Schistosoma mansoni | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
GSSG + NADPH + H+ | - |
Schistosoma mansoni | glutathione + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Schistosoma mansoni | thioredoxin + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SmTGR | - |
Schistosoma mansoni |
thioredoxin glutathione reductase | - |
Schistosoma mansoni |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000007 | - |
pH 7.0, 25°C, thioredoxin reductase activity | Schistosoma mansoni | auranofin | |
0.00001 | - |
pH 7.0, 37°C, glutathione reductase activity | Schistosoma mansoni | auranofin |