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Literature summary for 1.8.1.B1 extracted from

  • Alger, H.M.; Williams, D.L.
    The disulfide redox system of Schistosoma mansoni and the importance of a multifunctional enzyme, thioredoxin glutathione reductase (2002), Mol. Biochem. Parasitol., 121, 129-139.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a six-histidine tagged protein. Recombinant His-SmTGR, which would not have a penultimate selenocysteine when expressed in Escherichia coli, has no measurable activity in the insulin reduction assay Schistosoma mansoni

Inhibitors

Inhibitors Comment Organism Structure
auranofin
-
Schistosoma mansoni

Metals/Ions

Metals/Ions Comment Organism Structure
selenium selenocysteine protein Schistosoma mansoni

Organism

Organism UniProt Comment Textmining
Schistosoma mansoni Q962Y6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Schistosoma mansoni

Source Tissue

Source Tissue Comment Organism Textmining
adult thioredoxin glutathione reductase appears to be the major, if not the sole enzyme for these activities in adult worms, completely replacing thioredoxin reductase and glutathione reductase Schistosoma mansoni
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
Schistosoma mansoni 2-nitro-5-thiobenzoate + NADP+
-
?
GSSG + NADPH + H+
-
Schistosoma mansoni glutathione + NADP+
-
?
thioredoxin disulfide + NADPH + H+
-
Schistosoma mansoni thioredoxin + NADP+
-
?

Synonyms

Synonyms Comment Organism
SmTGR
-
Schistosoma mansoni
thioredoxin glutathione reductase
-
Schistosoma mansoni

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000007
-
pH 7.0, 25°C, thioredoxin reductase activity Schistosoma mansoni auranofin
0.00001
-
pH 7.0, 37°C, glutathione reductase activity Schistosoma mansoni auranofin