Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli Rosetta(DE3) | Pyrococcus furiosus |
expression in Escherichia coli Rosetta(DE3) | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
C146S | no activity against insulin disulfide, no DTNB-reducing activity | Pyrococcus furiosus |
C35S | strong activity as high as that of a wild type against insulin disulfide, 98% of residual activity. The activity of C35S against the reduction of DTNB was not decreased | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
gel filtration | Pyrococcus furiosus |
70000 | - |
gel filtration | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein-disulfide + NADPH + H+ | Pyrococcus furiosus | the enzyme is involved in a thioredoxin like system with thioredoxin reductase (PH1426). The redox potential of the redox protein is similar to that of thioredoxin from Escherichia coli and lower than that of glutathione | protein-dithiol + NADP+ | - |
? | |
protein-disulfide + NADPH + H+ | Pyrococcus furiosus OT-3 | the enzyme is involved in a thioredoxin like system with thioredoxin reductase (PH1426). The redox potential of the redox protein is similar to that of thioredoxin from Escherichia coli and lower than that of glutathione | protein-dithiol + NADP+ | - |
? | |
thioredoxin + NADP+ | Pyrococcus horikoshii | the enzyme forms a redox system with protein-disulfide oxidoreductase PhRP (PH0178) | thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin + NADP+ | Pyrococcus horikoshii DSM 12428 | the enzyme forms a redox system with protein-disulfide oxidoreductase PhRP (PH0178) | thioredoxin disulfide + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | O57917 | - |
- |
Pyrococcus furiosus OT-3 | O57917 | - |
- |
Pyrococcus horikoshii | O50134 | - |
- |
Pyrococcus horikoshii DSM 12428 | O50134 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
- |
Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme catalyzes the reduction of protein-disulfide oxidoreductase PhRP (PH0178) | Pyrococcus horikoshii | ? | - |
? | |
additional information | the enzyme catalyzes the reduction of protein-disulfide oxidoreductase PhRP (PH0178) | Pyrococcus horikoshii DSM 12428 | ? | - |
? | |
protein-disulfide + NADH + H+ | reduction activity for the disulfides in bovine insulin | Pyrococcus furiosus | protein-dithiol + NAD+ | - |
? | |
protein-disulfide + NADH + H+ | reduction activity for the disulfides in bovine insulin | Pyrococcus furiosus OT-3 | protein-dithiol + NAD+ | - |
? | |
protein-disulfide + NADPH + H+ | the enzyme is involved in a thioredoxin like system with thioredoxin reductase (PH1426). The redox potential of the redox protein is similar to that of thioredoxin from Escherichia coli and lower than that of glutathione | Pyrococcus furiosus | protein-dithiol + NADP+ | - |
? | |
protein-disulfide + NADPH + H+ | reduction activity for the disulfides in bovine insulin | Pyrococcus furiosus | protein-dithiol + NADP+ | - |
? | |
protein-disulfide + NADPH + H+ | the enzyme is involved in a thioredoxin like system with thioredoxin reductase (PH1426). The redox potential of the redox protein is similar to that of thioredoxin from Escherichia coli and lower than that of glutathione | Pyrococcus furiosus OT-3 | protein-dithiol + NADP+ | - |
? | |
protein-disulfide + NADPH + H+ | reduction activity for the disulfides in bovine insulin | Pyrococcus furiosus OT-3 | protein-dithiol + NADP+ | - |
? | |
thioredoxin + NADP+ | - |
Pyrococcus horikoshii | thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin + NADP+ | the enzyme forms a redox system with protein-disulfide oxidoreductase PhRP (PH0178) | Pyrococcus horikoshii | thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin + NADP+ | - |
Pyrococcus horikoshii DSM 12428 | thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin + NADP+ | the enzyme forms a redox system with protein-disulfide oxidoreductase PhRP (PH0178) | Pyrococcus horikoshii DSM 12428 | thioredoxin disulfide + NADPH + H+ | - |
? | |
[redox protein PhRP]-disulfide + NADH + H+ | redox protein PhRP from PH0178 | Pyrococcus furiosus | [redox protein PhRP]-dithiol + NAD+ | - |
? | |
[redox protein PhRP]-disulfide + NADH + H+ | redox protein PhRP from PH0178 | Pyrococcus furiosus OT-3 | [redox protein PhRP]-dithiol + NAD+ | - |
? | |
[redox protein PhRP]-disulfide + NADPH + H+ | redox protein PhRP from PH0178 | Pyrococcus furiosus | [redox protein PhRP]-dithiol + NADP+ | - |
? | |
[redox protein PhRP]-disulfide + NADPH + H+ | redox protein PhRP from PH0178 | Pyrococcus furiosus OT-3 | [redox protein PhRP]-dithiol + NADP+ | - |
? | |
[thioredoxin reductase from Escherichia coli]-disulfide + NADH + H+ | - |
Pyrococcus furiosus | [thioredoxin reductase from Escherichia coli]-dithiol + NAD+ | - |
? | |
[thioredoxin reductase from Escherichia coli]-disulfide + NADPH + H+ | - |
Pyrococcus furiosus | [thioredoxin reductase from Escherichia coli]-dithiol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 36000, SDS-PAGE | Pyrococcus horikoshii |
monomer | 1 * 26000, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
PH0178 | - |
Pyrococcus furiosus |
PH1426 | - |
Pyrococcus horikoshii |
PhRP | - |
Pyrococcus furiosus |
PhTrxR | - |
Pyrococcus horikoshii |
protein-disulfide oxidoreductase | - |
Pyrococcus furiosus |
thioredoxin reductase | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pyrococcus furiosus |
25 | - |
assay at | Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
retains full activity for over 1 h at 100°C | Pyrococcus furiosus |
100 | - |
retains full activity for over 1 h at 100°C | Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | in the sequence, PhTrxR appears to be the subfamily of flavoprotein reductase flavoprotein | Pyrococcus horikoshii | |
NADP+ | - |
Pyrococcus furiosus | |
NADP+ | - |
Pyrococcus horikoshii | |
NADPH | - |
Pyrococcus furiosus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in a thioredoxin like system with thioredoxin reductase (PH1426). The redox potential of the redox protein is similar to that of thioredoxin from Escherichia coli and lower than that of glutathione | Pyrococcus furiosus |